Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (38): 7071-7074.doi: 10.3969/j.issn.1673-8225.2011.38.010

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Biocompatibility of self-made calf deproteinized cancellous bone with rabbit cortical bone-derived osteoblasts

Meng Jia-rong1, Chen Chang-qing2, Zhang Shan-tao2, Lian Ke-jian2, Huang Li-xian2, Ding Zhen-qi2, Yin Xiao-feng2   

  1. 1Department of Pathology, Southeast Hospital of Xiamen University, Zhangzhou  363000, Fujian Province, China
    2Department of Orthopedics, Southeast Hospital of Xiamen University (Center of Orthopedics and Traumatology of PLA, the 175 Hospital of Chinese PLA), Zhangzhou  363000, Fujian Province, China
  • Received:2011-03-14 Revised:2011-04-13 Online:2011-09-17 Published:2011-09-17
  • Contact: Chen Chang-qing, Doctor, Associate chief physician, Department of Orthopedics, Southeast Hospital of Xiamen University (Center of Orthopedics and Traumatology of PLA, the 175 Hospital of Chinese PLA), Zhangzhou 363000, Fujian Province, China chencq1991@ hotmail.com
  • About author:Meng Jia-rong, Attending physician, Department of Pathology, Southeast Hospital of Xiamen University, Zhangzhou 363000, Fujian Province, China
  • Supported by:

    the Youth Talent Fund of Fujian Province, No. 2006F3146*; the Key Subject Fund of Nanjing Military Command during the Eleventh Five-year Plan, No. 06Z29*

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Abstract:

BACKGROUND: Heterogeneous deproteinized bone has the same structure with the host bone, and the antigen is low causing little or no host immune response.
OBJECTIVE: To observe the biocompatibility of self-made calf eproteinized cancellous bone with rabbit cortical bone-derived osteoblasts.
METHODS: Cells were isolated and cultured with enzyme digestion methods in vitro from the ulnar cortical bone of 3-month-old New Zealand white rabbits. Cells of the 3rd generation were attained for the following experiments. Rabbit cortical bone-derived osteoblasts were co-cultured with self-made calf eproteinized cancellous bone in vitro. Pure osteoblasts were used as controls. The cell growth, scaffold degradation and adherence condition were detected.
RESULTS AND CONCLUSION: The calf eproteinized cancellous bone was porous, and its pores were connected between each other. And with the culture time extended, cells grew and proliferated rapidly. On the 3rd day of co-culture, there were some cells adhered and spread on the scaffold; on the 6th day, cells were fully extended, showing long spindle; on the 10th day, cells secreted lots of extracellular matrix, presented with overlapping growth and were connected into the network. On the 9th and 12th day, the cell proliferation and secretion in the coculture group were superior to the control group (P < 0.05); Alkaline phosphatase activity and osteocalcin level were also higher in the coculture group than the control group (P < 0.05). The self-made calf deproteinized cancellous bone had good biological compatibility with rabbit cortical bone-derived osteoblasts.

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