Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (38): 7036-7040.doi: 10.3969/j.issn.1673-8225.2011.38.002

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Repair of long segment bone defects with a novel bone tissue engineering scaffold beta-tricalcium phosphate/calcium polyphosphate fiber/poly-L-lactic acid composite and bone marrow mesenchymal stem cells

Li Yi1, Liang Xiao-jun1, Wang Kun-zheng2, Chen Jun-chang2, Yang Tuan-min1, Tong Zhi-chao1   

  1. 1Red-Cross-Society Hospital of Xi’an City, Xi’an  710054, Shaanxi Province, China
    2Department of Orthopedics, the Second Hospital of Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China
  • Received:2011-04-11 Revised:2011-05-24 Online:2011-09-17 Published:2011-09-17
  • About author:Li Yi☆, Doctor, Associate chief physician, Red-Cross-Society Hospital of Xi’an City, Xi’an 710054, Shaanxi Province, China liyidoctor@163.com

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Abstract:

BACKGROUND: Bone tissue engineering scaffold beta-tricalcium phosphate/calcium polyphosphate fiber/poly-L-lactic acid (β-TCP/CPPF/PLLA) composite has good biocompatibility.
OBJECTIVE: To explore the effect of β-TCP/CPPF/PLLA scaffold and bone marrow mesenchymal stem cells (BMSCs) in repairing rabbit long segment bone defects.
METHODS: Forty New Zealand white rabbits were divided into BMSCs/β-TCP/CPPF/PLLA group (group A), pure β-TCP/CPPF/PLLA group (group B) and contrast group (group C) randomly. Animal models of long segment radius bone defect were established. The BMSCs/β-TCP/CPPF/PLLA were implanted into the right side of the radius defect (group A), while the pure β-TCP/CPPF/PLLA were implanted into the left side (group B) as control. We did nothing with the contrast group. The rabbits were sacrificed respectively after 4, 8, 12, 16 weeks and the X-ray film was performed at the same time to evaluate the repair effect in different groups.
RESULTS AND CONCLUSION: X-ray film showed there was a small quantity of clouding bony callus in defect region after 2 weeks in group A. Marked osteogenesis screenage could be seen after 4 weeks. The defect region was filled with neonate osseous tissue completely and the bone medullary cavity was completely recanalized during 12-16 weeks. The defect region was filled partly in group B and group C, while group B was significant than group C (P < 0.01). X-ray score revealed that group A was much higher than group B at any time (P < 0.01). The BMSCs/β-TCP/CPPF/PLLA composite were capable of repairing long segment bone defects.

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