Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (38): 7031-7035.doi: 10.3969/j.issn.1673-8225.2011.38.001

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Vascular endothelial growth factor transfected bone marrow mesenchymal stem cells cultured on bovine cancellous bone scaffold promote the formation of new vessels

Chen Yi1, Yin Xi-meng1, Han Ya-xin1, Wang Yan-feng1, Liu Dan-ping2, Tu Guan-jun1   

  1. 1Department of Orthopaedics, the First Affiliated Hospital of China Medical University, Shenyang  110001, Liaoning Province, China
    2Department of Orthopaedics, the First Affiliated Hospital of Liaoning Medical University, Jinzhou   121001, Liaoning Province, China
  • Received:2011-02-06 Revised:2011-03-06 Online:2011-09-17 Published:2011-09-17
  • Contact: Tu Guan-jun, Professor, Department of Orthopaedics, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China tgj188@gmail.com
  • About author:Chen Yi☆, Studying for doctorate, Physician, Department of Orthopaedics, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China chenyi0427163@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 81070971*

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Abstract:

BACKGROUND: After a small tissue engineering bone transplanted into animals, which can obtain enough nutrition by interstitial fluid penetration, but if a big one, it is insufficient by interstitial fluid penetration and must obtained by revascularization.
OBJECTIVE: To observe the ability of bone marrow mesenchymal stem cells (BMSCs) transfected with vascular endothelial growth factor (VEGF) expression vector to promote vascularization on tissue engineering bone.
METHODS: Bone defect model of the middle piece of both hand side ulnar bones of Japanese big ear rabbits was made. Transfected VEGF expression vector of autologous BMSCs to the protein of defatted bovine demineralized cancellous bone tissue engineering bone scaffold was implanted into left ulna defect as the experimental group. Autologous BMSCs combined with decalcifying, defatted and protein-free bovine cancellous bone scaffold of tissue engineering bone as the control group. At 12 weeks after operation, X ray photo observation, general specimen observation, hematoxylin-eosin staining and Masson staining histological section observation, MiFas picture analysis system quantitative analysis were performed.
RESULTS AND CONCLUSION: The continuous calluses were formed in ulnar bone defect between experimental and control groups. Tissue section was stained with hematoxylin-eosin staining and Masson staining, and observed by MiFas picture analysis system quantitative analysis, which showed a large number of new bones between experimental and control groups, but new vessels in experimental group was significantly more than that in control group (P < 0.01); vessles were thick and closed to new bones. It is indicated that BMSCs of transfected VEGF expression vector combined with tissue engineering bone and implanted into animals can obviously promote the formation of new vessels.

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