Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (35): 6647-6650.doi: 10.3969/j.issn.1673-8225.2011.35.044

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Association of the interleukin-16 gene polymorphisms with primary knee osteoarthritis susceptibility in female population of Guangxi Zhuang Autonomous Region

Luo Shi-xing1, Qin Xue2, Zhao Jin-min1, Su Wei1, Deng Yan2   

  1. 1Department of Trauma Orthopedics, 2Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2011-04-06 Revised:2011-07-18 Online:2011-08-27 Published:2011-08-27
  • Contact: Zhao Jin-min, Doctor, Professor, Department of Trauma Orthopedics, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China zhaojinmin@126.com
  • About author:Luo Shi-xing☆, Studying for doctorate, Attending physician, Department of Trauma Orthopedics, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China luosong888aaa@hotmail.com

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Abstract:

BACKGROUND: Current genetic researches show that the risk factors for knee osteoarthritis (OA) mostly related to interleukin (IL) 1, 6, 10 and other interleukin genes.
OBJECTIVE: To investigate whether the IL-16 gene polymorphisms is related to genetic susceptibility to primary OA in female.
METHODS: Blood samples were obtained from 71 primary OA patients defined by a Kellgren–Lawrence score of 2 or greater, and 85 controls of Guangxi Han female population. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect IL-16 gene (rs11556218, rs4072111, rs4778889) distribution between the two groups. Genotype and allele frequencies were calculated by SPSS, and Hardy-Weinberg equilibriums and non-conditional logistic regression was performed to evaluate the association between OA susceptibility and genotype using the SNPstats. 
RESULTS AND CONCLUSION: All genotype distributions of three single-nucleotide polymorphisms between the case and control group met Hardy-Weinberg equilibrium criteria (P>0.05). The results of non-conditional logistic regression analysis in codominant model showed that the T/T genotype of the rs11556218 might be a protective factor (OR=0.41, 95%CI=0.21-0.83, P=0.02) and the C/T genotype of the rs4072111 might be a risk factor (OR=2.36, 95%CI=1.19-4.68, P=0.03). It is indicated that the genetic polymorphisms of IL-16 gene may be associated with OA susceptibility in the Guangxi Han female population.

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