Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (35): 6571-6574.doi: 10.3969/j.issn.1673-8225.2011.35.026

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Constructing a rabbit model of necrosis of the femoral head by the combination of dexamethasone and lipopolysaccharide

Tian Li1, Liang Xiao-peng2, Tian Xiao-ye3, Yu Xin-chen4, Tian Jing4   

  1. 1Clinical Department, 2School of Optometry, Shenyang Medical College, Shenyang  110034, Liaoning Province, China; 3Jingkang Rehabilitation Pension Center, Tieling Economic Development Zone, Tieling  112000, Liaoning Province, China; 4Shenyang Jingkang Medical Group, Shenyang  110043, Liaoning Province, China
  • Received:2011-02-28 Revised:2011-02-28 Online:2011-08-27 Published:2011-08-27
  • Contact: Liang Xiao-peng, Master, Engineer, School of Optometry, Shenyang Medical College, Shenyang 110034, Liaoning Province, China Linan1956＠yahoo.com.cn
  • About author:Tian Li, Professor, Chief physician, Clinical Department, Shenyang Medical College, Shenyang 110034, Liaoning Province, China Linan1956@yahoo.com.cn
  • Supported by:

    the Foundation of Liaoning Educational Bureau, No. 2008-7-29*; the Funding Program of Shenyang Science and Technology Department, No. 1081233-1-00-03*

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Abstract:

BACKGROUND: An ideal animal model of necrosis of the femoral head is beneficial to the studies on the cause of disease, pathogenesis and treatment.
OBJECTIVE: To research the experimental result of necrosis of rabbit femoral head induced by the combination of dexamethasone and lipopolysaccharide.
METHODS: Thirty male New Zealand rabbits were divided into experimental group (20 rabbits) and control group (10 rabbits) randomly. Two injections of 10 μg/kg body lipopolysaccharide and three injections of 25 mg/kg dexamethasone were performed in the experimental group. Physiologic saline was injected in the control group.
RESULTS AND CONCLUSION: The CT scanning showed non-uniform of bone density of the femoral head in the experimental group. The indexes of Micro-CT bone metrological parameters were significantly lower in the experimental group than in the control group (P < 0.05). Bone cell lacunae, increased fat cells and thrombokinesis were observed in histopathological examination. The osteonecrosis rate of survived rabbits as well as the rate of empty bone lacunae was significantly lower in the experimental group than the control group. It is indicated that the combination of dexamethasone and lipopolysaccharide can induce an osteonecrosis model.

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