Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (35): 6567-6570.doi: 10.3969/j.issn.1673-8225.2011.35.025

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Static magnetic field reduces the toxic effect of metal ions on osteoblasts

Zhou Tong-hua1, Dai Min1, Cheng Xi-gao1, Zhan Ping1, Xiong Hao1, Zhou Wen-nan2, Yan Xiao-qing2   

  1. 1Department of Orthopedics, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China
    2Experimental Center of Engineering Mechanics, Engineering College of Nanchang University, Nanchang  330046, Jiangxi Province, China
  • Received:2011-03-29 Revised:2011-06-18 Online:2011-08-27 Published:2011-08-27
  • Contact: Dai Min, Department of Orthopedics, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China
  • About author:Zhou Tong-hua★, Master, Physician, Department of Orthopedics, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China butong2006@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30760255*; Interdisciplinary Fund of Nanchang University, No. Z03722*

Abstract:

BACKGROUND: Static magnetic field can promote the proliferation, differentiation and functional activity of osteoblasts.
OBJECTIVE: To investigate the toxic effect of metal ions Co2+, Cr3+ on osteoblasts under the static magnetic field.
METHODS: CoCl2 powder and CrCl3 powder were dissolved in the asepsis injecting water. Mouse calvarial osteoblasts (MC3T3-E1) were cultured with Co2+ and Cr3+ ions in different magnetic field of 1, 10, 100 mT. Cells only cultured with Co2+ and Cr3+ ions were taken as control group.
RESULTS AND CONCLUSION: Under the static magnetic field, osteoblasts showed more mature morphological features; the toxic effect of metal ions Co2+ and Cr3+ on osteoblasts reduced significantly; G2M (dividing phase) distribution ratio increased and G0G1 (dormancy stage) distribution ratio decreased; the A value tested by alkaline phosphatase activity in the three experiment groups up-regulated obviously. The static magnetic field in certain intensity could antagonize the toxic effect of metal ions Co2+ and Cr3+ on osteoblasts.

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