Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (34): 6375-6378.doi: 10.3969/j.issn.1673-8225.2011.34.025

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Preparation, characterization and cytotoxicity evaluation of polymeric nanomicelles based on modified chitosan

Jiang Gang-biao1, Lin Zhuan-tao1, Xu Xiao-peng2, Huang Zhi-jian2, Qi He-mei2   

  1. 1Department of Pharmaceutical Engineering, College of Natural Resource and Environment, South China Agricultural University, Guangzhou  510642, Guangdong Province, China
    2School of Life Sciences, Sun Yat-sen University, Guangzhou  510275, Guangdong Province, China
  • Received:2011-05-25 Revised:2011-07-18 Online:2011-08-20 Published:2011-08-20
  • About author:Jiang Gang-biao☆, Ph.D., Associate professor, Department of Pharmaceutical Engineering, College of Natural Resource and Environment, South China Agricultural University, Guangzhou 510642, Guangdong Province, China gbjiang@scau.edu.cn jgb3h@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 20874032*; the Science and Technology Plan of Guangdong Province, No. 2008B030301202*; the Combination Program of Guangdong Province, Ministry of Education, No. 2009B090300231*

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Abstract:

BACKGROUND: Chitosan, a natural polymer, can be chemically modified to prepare excellent biocompatible, biodegradable and low-cytotocitable polymeric nanomicelles, which has potent potential in the field of drug controlled release and tissue engineer.
OBJECTIVE: To graft a strong hydrophobic long chain, octadecyl alkoxy, onto succinyl chitosan to synthesize a novel kind of polymeric micelle with good stability and minute size, which can be utilized as drug carries or biomedical materials.
METHODS: Amphiphilic chitosan derivative MCS was obtained by introducing long hydrophobic chain to modify hydrophilic chitosan, which can form stable nanomicelles in aqueous solution. Octadecyl alkyl glycidyl ether was employed as hydrophobic group provider to react with N-succinyl chitosan.
RESULTS AND CONCLUSION: FTIR and 1H-NMR proved that the amphiphilic chitosan derivative (MCS) was synthesized successfully. The polymeric nanomicelles forming by MCS in water were 136-166 nm with negative charged surface. The values of critical micellar solubility of MCS micelles were in the ring from 1.67×10-3 to 3.35×10-3 g/L. The critical micellar solubility and size of the nannomicelles significantly increased with the increase of the substitute degree of hydrophobic group. The results of MTT assay illustrated that MCS can be a safe vehicle for drug.

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