Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (34): 6364-6367.doi: 10.3969/j.issn.1673-8225.2011.34.023

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A preliminary study on the histocompatibility of silk fibroin

Lu Yan1, Zhao Xia1, Shao Zheng-zhong2, Cao Zheng-bing2, Cai Li-hui1   

  1. 1Department of Otorhinolaryngology, Huashan Hospital, Fudan University, Shanghai  200040, China
    2Department of Macromolecular Science, Fudan University, Shanghai  200433, China
  • Received:2011-02-15 Revised:2011-04-22 Online:2011-08-20 Published:2011-08-20
  • Contact: Zhao Xia, Doctor, Professor, Department of Otorhinolaryngology, Huashan Hospital, Fudan University, Shanghai 200040, China zhaoxiaabc@126.com
  • About author:Lu Yan☆, Doctor, Attending physician, Department of Otorhinolaryngology, Huashan Hospital, Fudan University, Shanghai 200040, China luyan77@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30772400*; the Start-up Fund for Excellent Young Talents of Shanghai Medical College, No. 09L-12*

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Abstract:

BACKGROUND: As one kind of naturally occurring polymers, silk fibroin has been widely used in tissue engeering research, which can be made into different forms for application. Scientists find many difficulties in histocompatibility study of silk fibroin, because it is made of protein and biodegradable. And there are few reports on the study on histocompatibility of silk fibroin.
OBJECTIVE: To perform a preliminary in vivo study on the histocompatibility of silk fibroin.
METHODS: Porous silk fibroin scaffolds were buried into the subcutaneous part of the back of SD rats. At 4, 6, 8 weeks after operation, general observation and histological examination were performed.
RESULTS AND CONCLUSION: The wounds healed up well after operation. Only thin connective tissue wrap was found on silk fibroin scaffold. Macrophages were observed in tissue section, and fibroblasts and capillary vessels could grow into the pore spaces. We found the border of the scaffolds break down at 8 weeks postoperatively, while the inside part remained as it was. Porous silk fibroin scaffold shows good histocytes attachment and has good histocompatibility. More study should be made on the mechanism and product of degradation in vivo.

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