Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (34): 6306-6309.doi: 10.3969/j.issn.1673-8225.2011.34.009

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Preparation and characterization of keratinocyte growth factor loaded poly(lactic-co-glycolic) acid nanocapsules and their applications in reconstruction of tissue-engineered skin

Yang Bin1,2, Deng Li-huan1, Wang Zhao-yang3   

  1. 1 Plastic Surgery Institute, Peking Union Medical College, Beijing  100144, China
    2Guangzhou Medical College, Guangzhou  510182, China
    3Material Engineering School, South China University of Technology, Guangzhou 510640, Guangdong Province, China
  • Received:2011-03-05 Revised:2011-07-02 Online:2011-08-20 Published:2011-08-20
  • About author:Yang Bin☆, Professor, Plastic Surgery Institute, Peking Union Medical College, Beijing 100144, China ybdoctor_psh@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30772099*;Natural Science Foundation of Beijing, No. 7112111*

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Abstract:

BACKGROUND: Poly(lactic-co-glycolic) acid (PLGA) has good biocompatible and biodegradable properties.
OBJECTIVE: To prepare keratinocyte growth factor (KGF) loaded PLGA nanocapsules as the controlled-release drug carrier system suitable for skin-tissue engineering.
METHODS: The KGF-nanocapsules were prepared by double emulsion-solvent evaporation and freeze drying methods. Then, tissue-engineered technology was used to construct cell membrane. The morphology of nanocapsules as well as the growth and dermal connections of the cells were observed by scanning electron microscope (SEM) and inverted microscope. Mean diameter and particle size distribution were determined by particle size analyzer. The drug loading rate and encapsulation efficiency, as well as the release profile of KGF in the nanocapsules was evaluated indirectly by ultraviolet spectrometry and ELISA.
RESULTS AND CONCLUSION: Inverted microscope and SEM showed the nanocapsules spherical particles seemed to be smooth and uniform, distributed evenly on acellular dermal matrix (ADM) surface and linked tightly to ADM. The hair follicle stem cells grew well on the KGF-PLGA-ADM and formed clones. Both of them had a good compatibility. The drug-loading rate, encapsulation efficiency and KGF activity retention rate were (14.05±0.56)%, (59.86±2.38)% and (78.26±5.63)%, respectively. The released from nanocapsules appeared to be consistent with initial rapid-release and later slow- release. Accumulative release ratio was up to 75% in the continuous period of 30 days. It is indicated that KGF loaded PLGA nanocapsules prepared reasonably, which had good biocompatible and biodegradable properties and could be used to construct a new kind of tissue-engineered skin.

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