Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (34): 6285-6288.doi: 10.3969/j.issn.1673-8225.2011.34.004

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Vascularization on new tissue-engineered bone repair materials implanted into animals in vivo

Wang Wei1, Mi Lei2, Hou Guang-hui1, Wang Jian-long1   

  1. 1Department of Orthopaedics, Third Xiangya Hospital, Central South University, Changsha  410013, Hunan Province, China
    2Department of Orthopaedics, Second People's Hospital of Hunan Province, Changsha  410013, Hunan Province, China
  • Received:2011-02-05 Revised:2011-03-25 Online:2011-08-20 Published:2011-08-20
  • Contact: Wang Jian-long, Associate professor, Master’s supervisor, Department of Orthopaedics, Third Xiangya Hospital, Central South University, Changsha 410013, Hunan Province, China wangjl1972@sina. com.cn
  • About author:Wang Wei★, Master, Department of Orthopaedics, Third Xiangya Hospital, Central South University, Changsha 410013, Hunan Province, China wwtyy-006@163.com
  • Supported by:

    the Natural Science Foundation of Hunan Province, No. 09JJ3068*

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Abstract:

BACKGROUND: The research of bone tissue engineering achieved success based on growth factors, seed cells, vector scaffold, it demonstrates the better future of bone tissues reconstruction. However, clinical application is often not ideal. One of most important reason is that the absence of cell dependent disorder caused by vascular network of engineering bone graft.
OBJECTIVE: To observe the vascularization on new tissue-engineered bone repair materials implanted into radial defect of New Zealand rabbits.
METHODS: Balloon compression was prepared by the polylactic acid-polyglycolic acid copolymer (PLGA)/basic fibroblast growth factor (bFGF), then mixed with calcium phosphate cement (CPC), and combined with allogeneic bone marrow mesenchymal stem cells (BMSCs) cultured in vitro to prepare new tissue-engineered bone repair materials. A total of 60 adult New Zealand rabbits were used to establish animal experimental models of radial defect and randomly divided into two groups: Group A served as the experimental group which was transplanted with the new tissue-engineered bone repair materials. Group B served as the control group, which was transplanted with the mixed materials of composite BMSCs of PLGA and CPC. At 4, 8, 12 weeks after operation, the angiopoiesis at each period was observed by tissue cell morphology, nuclide bone scan and other methods.
RESULTS AND CONCLUSION: The results of histological observation under light microscope and nuclide bone scan showed experimental group was better than control group in vascularization. The results demonstrated that new tissue-engineered bone repair materials constructed by the PLGA-bFGF/CPC combined with BMSCs have a better vascularization in vivo.

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