Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (34): 6276-6280.doi: 10.3969/j.issn.1673-8225.2011.34.002

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Bioactivities of recombinant human bone morphogenetic protein 2-heparin artificial bone composite materials

Zhou Zhi-you1, He Shui-lian1, Sun Li1, Wang Ding-ding2, Wang Ju1   

  1. 1Institute of Bioengineering, College of Life Science and Technology, Jinan University, Guangzhou  510632, Guangdong Province, China
    2College of Life Science and Biological Pharmacy, Guangdong Pharmaceutical University, Guangzhou  510006, Guangdong Province, China
  • Received:2011-05-24 Revised:2011-07-07 Online:2011-08-20 Published:2011-08-20
  • Contact: Wang Ju, Doctor, Associate professor, Institute of Bioengineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, Guangdong Province, China wang_ju1688@yahoo.cn
  • About author:Zhou Zhi-you★, Studying for master’s degree, Institute of Bioengineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, Guangdong Province, China gikyo@yahoo.cn
  • Supported by:

    Guangdong Provinical Science and Technology Tackle Key Program, No. 2009B030801237*; the Enterprise Technological Special Correspondent Action Plan by Guangdong Province & Ministry of Education &Ministry of Science, No. 2009B090600134*

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Abstract:

BACKGROUND: Recombinant human bone morphogenetic protein 2 (rhBMP-2) has been applied to bone regeneration and repairing, but its ability to induce bone formation is limited because of its short half-life in vivo.
OBJECTIVE: To prepare rhBMP-2-heparin composite materials and to evaluate rhBMP-2 sustained delivery and osteoinductive effects.
METHODS: The effect of heparin on protecting rhBMP-2 from enzyme digestion was assayed by high-performance liquid chromatography. The materials were combined with rhBMP-2-heparin to produce rhBMP-2-heparin artificial bone composite materials, detecting the rhBMP-2 release effect by ELISA. Alizarin red staining was used to detect osteogenic ability. The ability to induce ectopic bone was detected through an in vivo mouse experiment.
RESULTS AND CONCLUSION: It is successful to prepare rhBMP-2 artificial composite materials with good sustained release effect of rhBMP-2, which can effectively induce osteocalcin and ectopic bone formation.

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