Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (31): 5843-5845.doi: 10.3969/j.issn.1673-8225.2011.31.034

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Subcutaneous implantation of human liver neoplasms in BALB/c naked mice and SCID mice

Bai Jian-hua1,2, Li Li2, Li Xiao-yan2, Zhu Xin-feng2, Chen Gang2   

  1. 1Kuming Medical University, 2Department of Hepatic- biliary- pancreatic Surgery, First People’s Hospital of Kunming, Kunming  650011, Yunnan Province, China
  • Received:2011-02-27 Revised:2011-06-14 Online:2011-07-30 Published:2011-07-30
  • Contact: Li Li, Doctor, Department of Hepatic-biliary- pancreatic Surgery, First People’s Hospital of Kunming, Kunming 650011, Shanxi Province, China
  • About author:Bai Jian-hua★, Master, Physician, Kuming Medical University, Kunming 650011, Yunnan Province, China Shanxi Province, China; Department of Hepatic-biliary- pancreatic Surgery, First People’s Hospital of Kunming, Kunming 650011, Yunnan Province, China jianhuabai3448@sina.com

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Abstract:

BACKGROUND: A good model of liver neoplasms is the basic for further diagnosis and treatment of liver carcinoma. There have been many types of models, but few of them are evaluated.
OBJECTIVE: To compare the tumor formation in two kinds of immune deficiency mice in which human liver neoplasms were implanted.
METHODS: CD133+ liver carcinoma stem cells were sorted from Huh7 hepatoma cells by immunomagnetic beads and 1×106 cells were implanted into the back skin of BALB/c naked mice and SCID mice. Within 20 days after implantation, the tumor formation and deaths of naked mice were investigated, and the maximal diameter of liver neoplasms was measured through the use of vernier caliper, and tumor with a diameter of 0.5 cm was considered successful.
RESULTS AND CONCLUSION: Among 24 BALB/c naked mice, tumor was successfully induced in 18 mice, and among 24 SCID mice, tumor was successfully induced in 16 mice. Chi-square test showed that there was no significant difference in tumor formation (P > 0.05), but significant difference in deaths exist (P < 0.05), between these two types of mice. These results showed that BALB/c mice are superior to SCID mice in establishment of subcutaneous implantation of liver carcinoma because they show low mortality and cost less.

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