Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (31): 5822-5826.doi: 10.3969/j.issn.1673-8225.2011.31.029

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Expressions of CD34，Delta-like-1 protein in hepatic regeneration tissue of rats after partial hepatectomy

Yang Zhi-yun1, Hou Yi-xin2, Wang Xian-bo1　   

  1. 1Department of Integrated Traditional Chinese and Western Medicine, Beijing Ditan Hospital, Capital Medical University, Beijing  100015, China
    2Beijing University of Chinese Medicine, Beijing  100029, China
  • Received:2011-05-06 Revised:2011-06-19 Online:2011-07-30 Published:2011-07-30
  • Contact: Yang Zhi-yun, Doctor, Associate chief physician, Master’s supervisor, Department of Integrated Traditional Chinese and Western Medicine, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China yangzhiyun6@ yahoo.com.cn Correspondence to: Wang Xian-bo, Chief physician, Doctoral supervisor, Department of Integrated Traditional Chinese and Western Medicine, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China wangxianbo638@ 163.com
  • About author:Yang Zhi-yun☆, Doctor, Associate chief physician, Master’s supervisor, Department of Integrated Traditional Chinese and Western Medicine, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China
  • Supported by:

    the National Natural Science Foundation of China, No. 30873423*; the Youth Science Research Program of Administration of Traditional Chinese Medicine of Beijing City, No. QN2010-7*

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Abstract:

BACKGROUND: When the liver affected severe damage or excalation, liver stem cells rapidly proliferated and differentiated into mature liver cells to repair liver tissue. However, liver regeneration is a complicated multiple signal molecular regulatory process and related genes and proteins that participated in regulation remain unclear.
OBJECTIVE: To investigate the expressions of CD34 and Delta-like-1 protein in rat liver regenerated tissues after partial hepatectomy.
METHODS: Rat hepatic regeneration models were established after hepatectomy. Expressions of ATPase, proliferating cell nuclear antigen (PCNA), CD34 and Delta-like-1 protein were detected by hematoxylin-eosin staining, enzyme-histochemistry and immunohistochemistry method at various time points.
RESULTS AND CONCLUSION: At the early stage of liver regeneration, the expression of ATPase was down-regulated, and the expressions of CD34 and Delta-like-1 protein were up-regulated. At the middle stage, the expression of ATPase was increased to the normal level, and the expressions of CD34 and Delta-like-1 protein were down-regulated. The expression of PCNA protein was continuously up-regulated. These suggest that the expressions of ATPase, CD34 and Delta-like-1 protein were different at different stages of hepatic regeneration, which means that different signal molecules were cooperated during the hepatic regeneration, and Notch-Delta signal pathway takes part in the pathogenesy.

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