Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (31): 5813-5816.doi: 10.3969/j.issn.1673-8225.2011.31.027

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Effects of self-made multi-organ preservation solution on nitric oxide synthase in cryopreserved rat testicle

Sun Wen, Zhang Jin-ping, Jie Fang, Xu Chao-chao, Jin Rong-pan, Fan Qi-qi, Qian Shen-lun, Ye Bo   

  1. Department of Clinical Medicine, School of Medicine, Shaoxing University, Shaoxing  312000, Zhejiang Province, China
  • Received:2011-04-14 Revised:2011-06-09 Online:2011-07-30 Published:2011-07-30
  • Contact: Zhang Jin-ping, Professor, Department of Clinical Medicine, School of Medicine, Shaoxing University, Shaoxing 312000, Zhejiang Province, China pjzxuan@126.com
  • About author:Sun Wen, Department of Clinical Medicine, School of Medicine, Shaoxing University, Shaoxing 312000, Zhejiang Province, China 911841162@163 com
  • Supported by:

    Science and Technology Innovation Program of College and University Students of Zhejiang Province in 2010, No.2010R426002*; Science and Technology Innovation Program of College and University Students of Shaoxing City in 2009, No.200912*; a grant from the First Batch Collage-level Excellent Teaching Team Construction Achievement Foundation of Shaoxing University, No.20097*

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Abstract:

BACKGROUND: During testis transplantation, cryopreservation and ischemia can lead to production of oxygen free radicals by testis, which damages testis tissue.
OBJECTIVE: To investigate the effects of self-made multi-organ preservation solution on nitric oxide synthase (NOS) in cryopreserved rat testicle.
METHODS: Rat testicle was cryopreserved by self-made multi-organ preservation solution and UW solution. At 24, 48, and 72 hours after cryopreservation, testicular tissue was taken to determine total antioxidant capacity and NOS activity.
RESULTS AND CONCLUSION: There was no significant difference in total antioxidant capacity and NOS activity in rat testicular tissue cryopreserved in the self-made multi-organ preservation solution versus UW solution (P > 0.05). These findings suggest that self-made multi-organ preservation solution can obviously reduce oxygen free radical damage in cryopreserved rat testicular tissue and its effects are equivalent to US solution. 

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