Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (26): 4751-4754.doi: 10.3969/j.issn.1673-8225.2011.26.001

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Epidural injection of collagenase into a goat model of lumbar disc degeneration

Zhu Wei-hong1, Zhu Hai-bo1, Ma Nan1, Xie Pin-liang2, Deng Hong-zhen2, Zhu Jian-min1   

  1. 1Department of Orthopedics, Xuhui Central Hospital, Shanghai  20003, China
    2Clincal Medical College of Jiangsu University, Zhenjiang  212003, Jiangsu Province, China
  • Received:2010-12-27 Revised:2011-03-15 Online:2011-06-25 Published:2011-06-25
  • Contact: Zhu Jian-min, Chief physician, Professor, Department of Orthopedics, Xuhui Central Hospital, Shanghai 200030, China
  • About author:Zhu Wei-hong, Attending physician, Department of Orthopedics, Xuhui Central Hospital, Shanghai 20003, China zhuweihong@medmail.com.cn
  • Supported by:

     the Science and Technology Research Plan of Shanghai Science and Technology Committee in 2008, No. 08411964900*

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Abstract:

BACKGROUND: The mechanism of epidural injection of collagenase to treat lumbar disc degeneration is still unclear.
OBJECTIVE: To assess the mechanism of epidural injection of collagenase in animal models of lumbar disc degeneration.
METHODS: Six adult sheep were excessively pressed with steel plate and L1/L2, L3/L4 segments were injected with 0.5 mL absolute alcohol to prepare lumbar disc degeneration models. Two weeks after epidural injection of collagenase, the sheep was killed to take the disc for electronic microscope observation.
RESULTS AND CONCLUSION: The electronic microscope showed that there were some fissures in the fibrous ring of the degenerated intervertebral disk, collagen fibers discs were dissolved, non-degenerative discs were unchanged. Collagenase can enter degenerated intervertebral disc and directly dissolve nucleus pulposus tissue.

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