Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (24): 4525-4529.doi: 10.3969/j.issn.1673-8225.2011.24.039

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Effect of cathepsin L/G on venous vascular wall in traumatic deep vein thrombosis rat models

Li Wen1, Hu Ji-hong2, Li Xing-guo2, Li Hong-kun2, Zhang Yu-bing2, Zhao Xue-ling2, Wang Bing2   

  1. 1Department of Cardiology, Yunnan Provincial People’s Hospital, Kunming  650032, Yunnan Province, China
    2Department of Orthopaedics, First Affiliated Hospital, Kunming Medical College, Kunming  650032, Yunnan Province, China
  • Received:2011-02-25 Revised:2011-05-11 Online:2011-06-11 Published:2011-06-11
  • Contact: Wang Bing, Doctor, Associate chief physician, Department of Orthopaedics, First Affiliated Hospital, Kunming Medical College, Kunming 650032, Yunnan Province, China wbdoctor@hotmail.com
  • About author:Li Wen, Associate chief physician, Department of Cardiology, Yunnan Provincial People’s Hospital, Kunming 650032, Yunnan Province, China akenika@hotmail.com Hu Ji-hong, Studying for doctorate, Associate professor, Department of Orthopaedics, First Affiliated Hospital, Kunming Medical College, Kunming 650032, Yunnan Province, China akenika168@gmail.com Li Wen and Hu Jing-hong contributed equally to this article.
  • Supported by:

    the National Natural Science Foundation of China, No. 30960389*, 81060151*; the Joint Funds of Yunnan Sci-Tech Department and Kunming Medical College, No. 2009cd159*

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Abstract:

BACKGROUND: At present, the basic molecular etiological mechanism and core regulatory network of deep vein thrombosis (DVT) remains uncertain, and there is not an ideal measure for early diagnosis of DVT.
OBJECTIVE: To study the underlying impact of cathepsin L/G in DVT rat model.
METHODS: DVT rat models (n = 50) were established by clamping both femoral vein in three different positions within 3 seconds with mosquito forceps and fixing with cast. According to different observation phases and biological situations of the femoral vein thrombosis, model rats were divided into thrombogenesis group, pre-thrombogenesis group and non-thrombogenesis group. An additional 10 normal rats served as control group. Femoral vein was obtained at corresponding time points to exact total RNA. After a gene chip-based screening, the data of gene expression were further dissected by real-time PCR. 
RESULTS AND CONCLUSUON: Gene chip hybridization analysis results demonstrated that differential expression of cathepsin L/G gene was significant among groups, and the expression was greatest in the thrombogenesis group, followed by pre-thrombogenesis and non-thrombogenesis groups, which was significantly greater than the control group (P < 0.05). Real-time PCR analysis results were consistent with gene chip hybridization analysis results. These indicate that DVT is associated with an increase in expression of cathepsin L/G in local venous vascular wall, and they may be candidate molecular markers for early diagnosis of deep vein thrombosis.

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