Abstract:

BACKGROUND: Suture method caused transient middle cerebral artery occlusion (MCAO) is the commonly used method to study focal cerebral ischemia. However, differences exist in cerebral ischemia models may lead to deviation of the experimental results.
OBJECTIVE: To analyze the types of cerebral ischemia in rats after MCAO method and its influential factors in different types.
METHODS: Referring to Longa’s method, 166 rats were prepared for MCAO models and scanned by MRI at 24 hours after the surgery. The rats were divided to cortex infarction group, subcortical infarction group and non-infarction group according to the scan results, and to analyze the insertion depth of suture in the surgery.
RESULTS AND CONCLUSION: The insertion depth were (19.9±0.9), (19.0±1.1) and (17.7±1.3) mm in the cortical infarction group, subcortical infarction group and non-infarction group, respectively. There was significant difference in the depth of suture insertion between the three groups of rats (P < 0.01). It is indicated that different types of cerebral infarction in rats may be due to the different insertion depths of suture. The insertion depth of suture is deeper into the blood vessel, and the risk of cortical infarction may be higher.