Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (24): 4395-4399.doi: 10.3969/j.issn.1673-8225.2011.24.007

Previous Articles     Next Articles

Establishment of non-alcoholic fatty liver model in mice

Wang Jun-jie1, Fang Hui-long1, Li Chun-wei1, 2, Chen Fu-chun1,Tang Wei-jun1, Jiang Xian-yong1, 2, Wu Hai-yan3, Chen Dong-lin1, 2   

  1. 1Xiangnan University, Chenzhou 423000, Hunan Province, China
    2Affiliated Hospital of Xiangnan University, Chenzhou 423000, Hunan Province, China
    3Chenzhou First People’s Hospital, Chenzhou 423000, Hunan Province, China
  • Received:2011-03-19 Revised:2011-05-16 Online:2011-06-11 Published:2011-06-11
  • Contact: Chen Dong-lin, Associate professor, Xiangnan University, Chenzhou 423000, Hunan Province, China; Affiliated Hospital of Xiangnan University, Chenzhou 423000, Hunan Province, China
  • About author:Wang Jun-jie★, Master, Lecturer, Xiangnan University, Chenzhou 423000, Hunan Province, China towjjemail@126.com Wang Jun-jie and Fang Hui-long contributed equally to this paper.
  • Supported by:

    a grant from Education Department of Hunan Province, No. 10C1246*; the Science and Technology Development Program of Chenzhou, No. 2008gl03*; the Natural Science Foundation of Hunan Province*; a grant from Key Laboratory of Cardio- cerebrovascular Natural Drugs of Hunan Province, Hunan Provincial Pharmacology Program of Xiangnan University*

PDF

969

Abstract:

BACKGROUND: Methods for inducing non-alcoholic fatty liver model (NAFLM) should be improved.
OBJECTIVE: To establish NAFLM in mice by high fat feeding and carbon tetrachloride.
METHODS: Kunming mice were randomly divided into three groups: control, high fat, and high fat plus carbon tetrachloride. Liver morphological and pathological changes were observed at 2, 4, 6 and 8 weeks. Serum levels of total cholesterol (TC), triglyceride (TG), alanine transaminase (ALT) and aspartate transaminase (AST) and hepatic TC, TG changes were determined.
RESULTS AND CONCLUSION: In high fat plus carbon tetrachloride group, inflammatory cell infiltration appeared in the mice liver after 2 weeks. There were a small amount of lipid droplets at 4 weeks, and steatosis emerged after 6 weeks. Hepatic fibrosis began to appear at 8 weeks. The serum levels of total TC, TG, ALT and AST, and hepatic TC, TG were significantly advanced in high fat plus carbon tetrachloride group compared with control group at 8 weeks (P < 0.05 or P < 0.01). The NAFLM can be established successfully by feeding with high fat food and injecting 5% carbon tetrachloride.

CLC Number: