Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (21): 3843-3846.doi: 10.3969/j.issn.1673-8225.2011.21.010

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Preparation and biocompatibility of new heterogeneous acellular nerve of squid

Fan Wei-jie1, Ding Zhi-qing2, Xu Xue-mei3   

  1. 1Department of Orthopedics, the Affiliated First Hospital, the University of South China, Hengyang  421001, Hunan Province, China
    2Department of Orthopedics, the First People’s Hospital of Zhuzhou, Zhuzhou  412000, Hunan Province, China
    3The Library of the University of South China, Hengyang  421001, Hunan Province, China
  • Received:2011-01-06 Revised:2011-04-15 Online:2011-05-21 Published:2011-05-21
  • About author:Fan Wei-jie☆, Doctor, Associate professor, Master’s supervisor, Department of Orthopedics, the Affiliated First Hospital, the University of South China, Hengyang 421001, Hunan Province, China flygreatman@126.com
  • Supported by:

    the Funding Program of Hengyang Science and Technology Committee, No. 2007KJ006*

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Abstract:

BACKGROUND: Acellular nerves of mammals have disadvantages: ①The diameter of myelinated fiber is small ; ②The cellular components are difficult to eliminate ; ③The cells are uneven when the acellular nerves are made into tissue engineering nerve.
OBJECTIVE: To prepare a new kind of squid acellular nerve and to explore its biocompatibility.
METHODS: The acellular nerves of squid were prepared by chemical methods (Haase chemical extraction). The effects of cells removed were estimated. The integrity of the nerve fiber tube and Schwann cell basilar membrane was observed. The compatibilities of acellular nerves of squid and cells were detected by MTT assay. Its histocompatibility was observed by subcutaneous implantation experiment.
RESULTS AND CONCLUSION: The cells and myelin of acellular squid nerve prepared by Haase chemical extraction had been cleared completely, and the integrity of the nerve fiber tube and Schwann cell basilar membrane had been remained, and the tube of nerve fiber were large. Subcutaneous implantation results indicated that inflammatory cells expressed around the material at 1 week, 2 weeks after implantation, and thin fibrous connective tissue capsule appeared around the material at 4 weeks after implantation, and few inflammatory cells expressed. The compatibility of xenogeneic cell and the compatibility of xenogeneic tissue were good.

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