Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (21): 3839-3842.doi: 10.3969/j.issn.1673-8225.2011.21.009

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Biocompatibility of energied copolyesters coating for magnesium alloy evaluated by cell culture assay

Pan Feng1, 2, Bai Shu-ling1   

  1. 1Department of Anatomy, 2Department of Biological Materials, China Medical University, Shenyang  110001, Liaoning Province, China
  • Received:2011-02-09 Revised:2011-03-15 Online:2011-05-21 Published:2011-05-21
  • About author:Pan Feng☆, Associate professor, 1Department of Anatomy, 2Department of Biological Materials, China Medical University, Shenyang 110001, Liaoning Province, China pandaifeng@sina.com
  • Supported by:

    the National Natural Science Foundation of China, No. 81000791*; Liaoning Provincial Project in Higher Education Institutions of Educational Committee, No. L2010580*

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Abstract:

BACKGROUND: Compared with polylactic acid (PLA), polyglycolic acid (PGA) and PLA-PGA, 3-hydroxybutyrate-co-3 - hydroxyhexanoate copolyesters (PHBHHx) has structural diversity, good biocompatibility, biodegradability and hot-working character.
OBJECTIVE: To evaluate the biocompatibility of PHBHHx at different ferulic acid mass concentration by cell culture assay.
METHODS: Acetic ether was used to prepare films with 0 (control group), 50, 100, 130, 150 g/L ferulic acid PHBHHx and the appearance of films was observed. MTT test, fluorescence microscope and scanning electron microscope were used to assess the growth of bone marrow mesenchymal stem cells surface.
RESULTS AND CONCLUSION: PHBHHx with 50 g/L ferulic acid showed a lucent film without white crystalline precipitates, but obvious white crystalline precipitates distributed in films formed by PHBHHx with 100, 130, 150 g/L ferulic acid. MTT test indicated PHBHHx with 50, 100 g/L ferulic acid could better promote the growth of bone marrow mesenchymal stem cells. The adhension of bone marrow mesenchymal stem cells in the surface of PHBHHx with 50g/L ferulic acid could be seen under fluorescence microscope and scanning electron microscope. PHBHHx with 50 g/L ferulic acid is the most appropriate coating for magnesium alloys.

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