Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (12): 2179-2182.doi: 10.3969/j.issn.1673-8225.2011.12.022

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Nano-fiber-mediated paclitaxel combined with doxorubicin for sequential chemotherapy of SHg-44 glioma

Wang Zhan-feng1, 2, Sun Yu-qin1, Han Hai-ling1, Xu Xiu-ling3, Jing Xia-bin3   

  1. 1Second Department of Brain Surgery, Affiliated Hospital of North China University, Jilin 132011, Jilin Province, China
    2Jilin University, Changchun  132011, Jilin Province, China
    3Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun  130022, Jilin Province, China
  • Received:2010-09-11 Revised:2011-01-17 Online:2011-03-19 Published:2011-03-19
  • About author:Wang Zhan-feng☆, Doctor, Associate professor, Second Department of Brain Surgery, Affiliated Hospital of North China University, Jilin 132011, Jilin Province, China 1206hhl@sina.com
  • Supported by:

    National Natural Science Foundation of China, No. 20904002*; Scientific Research Foundation for Distinguished Young Scientists of Technology Bureau of Jilin Province, No. 20090193*; the "Eleventh Five-Year" Major Scientific and Technological Research of Education Department of Jilin Province, No. 2009139*; Scientific Research Foundation for Distinguished Young Scientists of Jilin, No. 20090606*

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Abstract:

BACKGROUND: Nano-fiber technology can simultaneously support many kinds of drugs, avoid the limitation of blood-brain barrier and achieve sequential combination chemotherapy of glioma.
OBJECTIVE: By means of emulsion-electrospinning method, both paclitaxel (PTX) and doxorubicin (DOX), were successfully loaded into poly (ethylene glycol)-b-poly (L-lactic acid) copolymer (PEG-PLA) nanofiber to realize sequential chemotherapy and to explore the effect and mechanism of SHg-44 glioma mediated by PEG-PLA nano-fiber.
METHODS: The experiment was divided into 4 groups: 1640 culture medium group, 1% DOX group, 1% PTX group, and 5% (DOX + PTX) group. The release in vitro of PTX and DOX was measured by high performance liquid chromatography (HPLC). The inhibition ratio of SHg-44 glioma cell proliferation on nano-fiber mediated PTX and DOX sequential chemotherapy was determined by MTT assay. Flow cytometry was used to detect the SHg-44 glioma cell apoptosis induced by PTX and DOX sequential chemotherapy.
RESULTS AND CONCLUSION: Nano-fiber mediated PTX and DOX sequential chemotherapy had significant growth inhibition and pro-apoptotic effect to SHg-44 glioma cell, and the effect was better than individual drug application. It is indicated that PEG-PLA nano-fiber as a drug carrier can increase the effect of PTX and DOX on SHg-44 glioma cell proliferation and induction of apoptosis.

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