Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (12): 2175-2178.doi: 10.3969/j.issn.1673-8225.2011.12.021

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Feasibility of flexor tendon allograft cryopreserved with vitrification

Hu Cheng-dong1, Liu Xi2, Zhang Bo-xun3, Wei Min3, Zhao Liang1, Zhou Yu-jun1, Chen Huai-zhi1, Wang Fei1, Li Dong-feng1   

  1. 1Department of Orthopaedics, 2Department of Immunology, Handan City Central Hospital, Handan  056001, Hebei Province, China; 3Department of Orthopaedics, General Hospital of Chinese PLA, Beijing   100853, China
  • Received:2010-10-06 Revised:2010-11-12 Online:2011-03-19 Published:2011-03-19
  • About author:Hu Cheng-dong☆, Doctor, Associate chief physician, Department of Orthopaedics, Handan City Central Hospital, Handan 056001, Hebei Province, China hcd111111@163.com
  • Supported by:

    Science and Technology Research and Developmental Program of Handan City, Hebei Province, No.0828108259-3*

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Abstract:

BACKGROUND: The main goal to process tendon allograft was to reduce immunogenicity and to retain structure and activity of tendon at the same time. Cryopreservation was commonly used in clinic, which was complicated, time-consuming, and activity of tendon was lower.
OBJECTIVE: To preserve flexor tendon in chicken by vitrification, and to explore its feasibility being used as allograft.
METHODS: Far flexor digitorum profundus tendon of adult Leghorn metatarsophalangeal joint was cut off, and randomly divided into two groups with number table: vitrified tendon group and autologous tendon group. Vitrified tendon allograft and autologous tendon was transplanted, respectively. At 2, 4, 8, 12, 16 weeks after operation, changes of tendon graft in morphosis, hydroxyproline content and mechanical properties and so on were observed.
RESULTS AND CONCLUSION: The vitrified tendon transplantataion group was found light immunologic rejection after implantation in early stage, but which didn’t affect tendon healing and reconstruction. There was middling level adhesion around tendon in two groups. The content of hydroxyproline in the middle of tendon grafts decreased before 8 weeks, and then gradually increased. However, the content of hydroxyproline in tendon stoma was gradually increased with time. There was no significant difference between two groups. Ruptured intensity and elasticity modulus of the vitrified tendon transplantation group were lower than autologous tendon group (P < 0.05) in the part of tendon stoma before 8 weeks, but there was no significant difference after 12 weeks. And there was no significant difference between two groups of the middle of tendon grafts in biomechanics property. The vitrified tendon allograft has good biological viability and low immunogenicity, which could be as well tendon allograft.

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