Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (12): 2171-2174.doi: 10.3969/j.issn.1673-8225.2011.12.020

Previous Articles     Next Articles

Cytocompatibility of electro-spinning nano-fibrous scaffolds for skeletal muscle tissue engineering

Liang Shuang1, Li Quan1, Tang Xiu-fa2, Feng Yang1, He Deng-qi3   

  1. 1State Key Laboratory of Oral Disease, Chengdu  610041, Sichuan Province, China
    2Department of Head and Neck Oncology, West China College of Stomatology, Sichuan University, Chengdu   610041, Sichuan Province, China
    3Department of Stomatology, the Second Affiliated Hospital of Lanzhou University, Lanzhou   730030, Gansu Province, China
  • Received:2010-10-15 Revised:2010-11-21 Online:2011-03-19 Published:2011-03-19
  • Contact: Tang Xiu-fa, Professor, Doctoral supervisor, Department of Head and Neck Oncology, West China College of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China
  • About author:Liang Shuang★, Studying for master’s degree, State Key Laboratory of Oral Disease, Chengdu 610041, Sichuan Province, China nancy1986xx@126.com
  • Supported by:

    Doctor Subject Foundation of the Ministry of Education of China, No. 20050610077*; the National Natural Science Foundation of China, No. 30872890*

PDF

513

Abstract:

BACKGROUND: Some scholars reported that biodegradable collagen plate or poly L-lactic acid (PLLA), poly glycolic acid (PGA), PLLA/PGA copolymer, serve as scaffold materials of skeletal muscle tissue engineering. But these materials have their own advantages and disadvantages, and still can not fulfill the needs of skeletal muscle tissue engineering.
OBJECTIVE: To study the feasibility of the electro-spinning nano-fibrous membrane as scaffolds for skeletal muscle tissue engineering.
METHODS: The rat myoblasts at the third passage were cultured with extracts obtaining from different kinds of electro-spinning nano-fibrous membranes (7 groups), with an extractant of F12 media containing 20% bovine calf serum. The adhesion and growth of myoblasts on the nano-fibrous membrane were examined with MTT and scanning electron microscopy methods.
RESULTS AND CONCLUSION: The absorbance value between any two groups has no statistically significant difference compared with control group (P > 0.05), while obvious statistical differences of adhesion rate lay in different groups of biomaterials (P < 0.05). The same result lies in the scanning electron microscopy image. A great quantity of myoblasts adhered to the 70% PLA + 20% silk fibroin + 10% collagen group. These myoblasts are spindle-shaped, regularly arranged, and connecting to adjacent cells. In other groups, the myoblasts are less and irregular, much more like myoblasts in recession. The electro-spinning nano-fibrous membrane has no toxicity and no bad effect on the growth of myoblasts. They adhere well on the membrane, especially on the 70% PLA + 20% silk fibroin + 10% collagen group.

CLC Number: