Abstract:

BACKGROUND: Frozen dried bone allograft is a good material of bone transplantation. But in the preparation procedure, some part of natural activity will be lost and osteogenic activity descent. To improve osteogenic activity of frozen dried bone, new materials are hoped to invent and used in clinic.
OBJECTIVE: To evaluate biocompatibility of strontium incorporated frozen dried bone in vivo.
METHODS: The rat frozen dried bone was prepared according to a standard procedure, then the bone was immersed into strontium chloride to prepare the rat strontium incorporated frozen dried bone. Totally 27 healthy adult SD rats were randomly assigned to 3 groups. Rats were prepared for thigh muscle pouches models, and left and right sides were both operated. Strontium incorporated frozen dried and normal frozen dried bones were transplanted into thigh muscle pouches as experimental and control, respectively. The both sides of thigh muscle pouches without implant served as blank control. Three rats were sacrificed from each group at 4, 8 and 12 weeks after operation to undergo gross, histological observation, and the inflammatory infiltration and fibrosis were scored.
RESULTS AND CONCLUSION: Strontium concentration in frozen dried bone achieved 4.2% through soaking method treatment. Inside the bone, strontium concentration was even. The inflammatory infiltration and granulation tissues and angiogenesis were seen in the experimental and control groups. The inflammatory infiltration was alleviated gradually with time prolonged and the surrounding fibrous tissues increased. Inflammatory reactions in the blank control group were alleviated gradually and less fibrosis. The scores of inflammatory infiltration and fibrosis of the experimental and control groups were significant different from that of the blank control group (P < 0.05). Strontium incorporated frozen dried bone can be made by ion-exchange method, which has good biocompatibility similar to normal frozen dried bone.