Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (12): 2141-2145.doi: 10.3969/j.issn.1673-8225.2011.12.013

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Biocompatibility of acellular natural bone matrix with induced osteoblasts in vitro

Li Kang-jie1, Sun Shu2   

  1. 1Second Department of Orthopaedics, Affiliated Hospital of Yanbian University, Yanji  133000, Jilin Province, China
    2Department of Pathology, Yanbian University School of Medicine, Yanji  133000, Jilin Province, China
  • Received:2010-09-15 Revised:2010-11-02 Online:2011-03-19 Published:2011-03-19
  • Contact: Sun Shu, Doctor, Professor, Department of Pathology, Yanbian University School of Medicine, Yanji 133000, Jilin Province, China
  • About author:Li Kang-jie☆, Studying for doctorate, Associate professor, Second Department of Orthopaedics, Affiliated Hospital of Yanbian University, Yanji 133000, Jilin Province, China likangjie2010@sina.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 81000791*

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Abstract:

BACKGROUND: The repair requirements of histology and immunology can be met by acellular bone matrix treated by TritonX-100. The test cells will be able to grow nicely on the surface of test materials, which will benefit further evaluating them in animals in vivo.
OBJECTIVE: To evaluate the biocompatibility of acellular natural bone matrix prepared with TritonX-100 to osteoblasts by cell culture assay.
METHODS: The third passage of bone marrow stromal stem cells were induced to differentiate into osteoblasts, then the identified osteoblasts were seeded on surface of acellular natural bone matrix prepared with TritonX-100 and calcium hydroxyapatite. Alkaline phosphatase vitality was measured by alkaline phosphatase detection kit and the growth of osteoblast-like cells on surface of the material was observed with scanning electron microscopy.
RESULTS AND CONCLUSION: Alkaline phosphatase detection kit analysis indicated that acellular natural bone matrix prepared with TritonX-100 were able to promote the growth of osteoblast-like cells compared with calcium hydroxyapatite after 48 hours of culture. Scanning electron microscopy displayed that osteoblast-like cells on the surface of acellular natural bone matrix aggregated together and grew as three-dimensional style. In vitro experimental proved good biocompatibility of osteoblast-like cells and acellular natural bone matrix.

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