Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (12): 2109-2112.doi: 10.3969/j.issn.1673-8225.2011.12.005

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Osteoinductive calcium phosphate ceramics for in vivo construction of tissue engineered bone in adipose tissue

Yao Jin-feng1, Li Xiao-yu2, Wang Ai-jun1, Liang Rui1, Bao Chong-yun3, Chen Zhi-qing3   

  1. 1Department of Stomatology, Shenzhen Second People’s Hospital, Shenzhen  518035, Guangdong Province, China
    2Department of Oral and Maxillofacial Surgery, The People’s Hospital of Baoan District, Shenzhen  518101, Guangdong Province, China
    3State Key Laboratory of Oral Diseases, West China School/ Hospital of Stomatology, Sichuan University, Chengdu  610041, Sichuan Province, China
  • Received:2010-10-10 Revised:2010-12-24 Online:2011-03-19 Published:2011-03-19
  • Contact: Liang Rui, Chief physician, Department of Stomatology, Shenzhen Second People’s Hospital, Shenzhen 518035, Guangdong Province, China
  • About author:Yao Jin-feng☆, Doctor, Attending physician, Department of Stomatology, Shenzhen Second People’s Hospital, Shenzhen 518035, Guangdong Province, China jinfeng-yao@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30672337*

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Abstract:

BACKGROUND: For constructing tissue engineered bone, the choice of non-osseous tissue is of great importance. Most early studies adopt the muscle as the site of constructing ectopic bone graft, but its location is deep, available area is small, the operation is complex, thus it is not conducive to clinical practice.
OBJECTIVE: The study is aimed to investigate the feasibility of constructing tissue engineered bone graft with osteoinductive calcium phosphate ceramics in non-osseous tissue by means of in vivo bone tissue engineering method.
METHODS: The muscle tissue and adipose tissue on the back of dogs were taken as constructed area, and implanted with osteoinductive calcium phosphate ceramics to construct in vivo tissue engineered bone graft. The specimens were harvested at 4, 8, 12 and 16 weeks post operation, for gross observation, single photon emission computerized tomography and histological studies. The osseous tissue formation at different times in two non-osseous sites was compared and the new bone grafts in subcutaneous fat and intramuscular implantations were evaluated.
RESULTS AND CONCLUSION: The tissue engineered bone graft could be constructed not only in muscle but also in subcutaneous fat. The new bone appeared in the muscle earlier than that in the fat, the amount of new bone mass increased with time in two non-osseous tissues, and the difference between in muscle and fat decreased. Vital autogenous bone graft can be constructed in the muscle and fat tissues by means of bone tissue engineering method in vivo. Compared to that in muscle, constructing bone graft in subcutaneous fat could have brighter prospect to clinical application due to widely area and superficial location.

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