Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (12): 2105-2108.doi: 10.3969/j.issn.1673-8225.2011.12.004

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Hyaluronate sodium and peripheral blood mesenchymal stem cells compound for repairing cartilage defects

Wei Bin1, Wang Dong-lai2, Feng Jian-gang2   

  1. 1Department of Orthopaedfics, the 252 Hospital of Chinese PLA, Baoding  071000, Hebei Province, China
    2Department of Orthopaedics, Fourth Hospital of Hebei Medical University, Shijiazhuang  050011, Hebei Province, China
  • Received:2010-10-21 Revised:2011-01-04 Online:2011-03-19 Published:2011-03-19
  • Contact: Feng Jian-gang, Professor, Chief physician, Master’s supervisor, Department of Orthopaedics, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei Province, China 2006fjg@163.com
  • About author:Wei Bin★, Master, Department of Orthopaedfics, the 252 Hospital of Chinese PLA, Baoding 071000, Hebei Province, China w_binaaaa@163.com
  • Supported by:

    a grant by Hebei Provincial Health Department, No.06103*

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Abstract:

BACKGROUND: In recent years, peripheral blood mesenchymal stem cells (MSCs) have been greatly investigated, but no study reports its application for the repair of cartilage defects.
OBJECTIVE: To inject the compound of mobilized peripheral blood derived MSCs and hyaluronate sodium into articular cavity, and to investigate the feasibility of applying the compound to repair articular cartilage defect.
METHODS: Two-month aged rabbits were used to isolate MSCs from peripheral blood after granulocyte colony-stimulating factor and stem cell factor mobilization. Primary and passage culture was to be continued. Taking hyaluronate sodium as the carrier materials, cell suspension was prepared by mixing with passage 3 MSCs. The full-thickness cartilage defects were created on both medial and lateral femoral condyles on every knee joint of three-month aged rabbits. Left knees were taken as the experimental group, injecting cell suspension (containing 0.5 mL hyaluronate sodium) into articular cavity, while right knees as control group, injecting hyaluronate sodium 0.5 mL once a week, for continuous three times. Twelve weeks later, rabbits were killed to observe the state of repairing the defects, the type of new tissues and the articular immune response.
RESULTS AND CONCLUSION: ①The appearance of peripheral blood derived MSCs was similar with bone marrow derived MSCs. When they were isolated from peripheral blood, primary cells could be cultured. ②After injection, no inflammatory reaction and infection were seen, joint motion was well. ③After injection for 12 weeks, the defects in experiment group were partially stuffed by cartilage-like tissues. The surface in defects was smooth, but parts of the boundary between repair tissue and surrounding could be seen. Superficial layer’s fibrosis was more obviously. Effect of articular cartilage repair in the experimental group was better than the control groups. Peripheral blood derived MSCs mixed with hyaluronate sodium possess the capability of repairing articular cartilage defect.

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