Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (12): 2091-2094.doi: 10.3969/j.issn.1673-8225.2011.12.001

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Poly(lactic-co-glycolic acid)/tricalcium phosphate scaffolds prepared by rapid prototyping technology for the repair of radial defects in rabbits

Sun Liang1, Xiong Zhuo2   

  1. 1Third Hospital of Hebei Medical University, Shijiazhuang  050000, Hebei Province, China
    2Department of Mechanical Engineering, Tsinghua University, Beijing  100084, China
  • Received:2010-10-26 Revised:2011-01-05 Online:2011-03-19 Published:2011-03-19
  • About author:Sun Liang☆, Doctor, Associate professor, Associate chief physician, Third Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China sunpan73@msn.com
  • Supported by:

    the National 863 Project, No. 715-009-0150*; a grant by the National High-Technique Plan  for New Materials During the Ninth Five-Year Plan, No. 009-0160*; Key Program of Military Medical Research Fund, No. 01Z079*

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Abstract:

BACKGROUND: The ideal bone repair materials must be biocompatible and absorbable, have the porosity that is conducive to the vascularization and rapidly replaced by new tissue, as well as a three-dimensional structure similar to bone tissue.
OBJECTIVE: To test the effect of poly(lactic-co-glycolic acid)/tricalcium phosphate (PLGA/TCP) scaffolds prepared by rapid prototyping technology loaded with bone morphogenetic protein in the repair of radial defect in rabbits.
METHODS: The lactic acid/glycolic acid copolymer dissolved in 1,4-dioxane and mixed with calcium phosphate powder to prepare a liquid slurry, then placing into a biomaterial rapid prototyping machine TissFormTM, thus a cylindrical artificial bone carrier at the diameter of 5 mm and length of 15 mm was obtained. According to the standard of 15 mg in each material, active artificial bone materials were prepared by use of pre-wet, negative pressure of bone morphogenetic protein, freeze-drying. Twenty New Zealand rabbits were used to prepare a 15-mm radial defect, which was treated by the implantation of active artificial bone loaded with and without bone morphogenetic protein. Then results of imaging, histology, scaffolds degrade rates and bone mineral density was appraised to examine the repairing effects of the scaffolds.
RESULTS AND CONCLUSION: At 12 weeks, all defects in the experimental group were radiographically repaired. New bony callus was observed to connect with the defect ends and then mould, the scaffold was nearly completely absorbed, and no significant differences were observed compared with control group. PLGA/TCP scaffolds prepared by rapid forming technology loaded with bone morphogenetic protein can effectively repair the 15-mm long bone defects in rabbits, its degradation rate well matched the bone formation rate.

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