Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (9): 1527-1530.doi: 10.3969/j.issn.1673-8225.2011.09.003

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Receptor pathway of lumbar facet joint and lamina muscle attachment points 

Zhang Yun-hai1,2, Geng Zhu-sheng1,2, Lei Ling1, Cui Ji-zheng1,2, Zhang Xiao-bao1, Yang Cong-ying3, Li Yu4, Xu Xin-tang5   

  1. 1Department of Anesthesiology, 2Department of Pain, 3Department of Pathology, 4Clinical Laboratory Center of Lianyungang, 5Department of Radiology, First People's Hospital of Lianyungang, Lianyungang  222002, Jiangsu Province, China
  • Received:2010-09-19 Revised:2010-10-15 Online:2011-02-26 Published:2011-02-26
  • About author:Zhang Yun-hai, Chief physician, Department of Anesthesiology, Department of Pain, First People's Hospital of Lianyungang, Lianyungang 222002, Jiangsu Province, China zyh1957423@sina.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30271260*; the Program of Lianyungang Science and Technology Bureau, No. SH0408*, SH0507*, SH0905*

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Abstract:

BACKGROUND: Drug administration and silver needle heat conductive treatment through receptor pathway of lumbar facet joint and lamina muscle attachment points can achieve good effect in the treatment of discogenic low back pain, but the pathway remains unclear.
OBJECTIVE: To observe the receptor pathway of lumbar facet joint and lamina muscle attachment points morphologically in the rabbits.
METHODS: A total of eight rabbits were randomly divided into 2 groups. Fluorescent riboflavin was injected into the bilateral lumbar facet and lamina muscle attachment points under CT guidance. At 18 and 36 hours after injection, consecutive frozen sections were made in T1-L5 spinal ganglia, cervical, thoracic sympathetic ganglion, inferior mesenteric ganglion, brain, cerebellum, thalamus, brain stem, spinal cord, internal organs, blood vessels, skin, synovial membrane of lumbar facet joint, tendon of lamina muscle attachment, disc, testes and ovaries, etc. Nerve cells and nerve endings labeled by fluorescence were observed under fluorescence microscope and hematoxylin-eosin staining. The exposure time was recorded.
RESULTS AND CONCLUSION: Nerve cells labeled by fluorescence were observed in hypothalamus, neck, thoracic sympathetic ganglion, T12-L5 spinal ganglia, gastric mucosa, the intestinal mucosa and submucosa and the inferior mesenteric ganglion 18 hours after injection. Fluorescent-intensive area was observed in frontal horn and posterior horn of spinal cord, brain, cerebellum, annulus fibrosus disci intervertebralis, nucleus pulposus of lumbar intervertebral disc, lumbar synovial membrane between the skeletal muscle membrane, inside and outside membrane of trachea, inside membrane of lung, internal and external membrane of cardiac muscle, glomeruli, tubules, wall of gallbladder, inside and outside membrane of fallopian tube, ovary, inside and outside membrane of uterus, testis, capillary loops of skin and wall of mesenteric artery. Lots of lymphocyte were seen in the spinal ganglia under hematoxylin-eosin staining, fluorescent-intensive areas were similar to fluorescence microscope, but the exposure time was significantly reduced (P < 0.01). At 36 hours after injection, labeled cells in spinal ganglion were significantly reduced, and exposure time was significantly lengthened (P < 0.01). Fluorescence-intensive areas were the same, but the exposure time in some sites was significantly lengthened (P < 0.01), significantly decreased at some sites (P < 0.01). Receptor pathway network of lumbar facet joint and lamina muscle attachment points in the rabbit were composed by sympathetic ganglia and postganglionic neurons, intestinal ganglion cells, spinal ganglia, parasympathetic neuron in peripheral and part neurons in the hypothalamus.

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