Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (8): 1459-1462.doi: 10.3969/j.issn.1673-8225.2011.08.031

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Mechanisms of cationic liposome-mediated gene transfer

Wang Bing1, 2, Zhang Shu-biao2, Zhou Ji-ti1, Zhao Bu-diao2, Yang Bao-ling2, Cui Shao-hui2, Zhao Yi-nan2   

  1. 1School of Environmental and Biological Science, Dalian University of Technology, Dalian   116024, Liaoning Province, China
    2Key Laboratory of State Ethnic Affairs Commission-Ministry of Education, Dalian Nationalities University, Dalian   116600, Liaoning Province, China
  • Received:2010-09-16 Revised:2010-12-17 Online:2011-02-19 Published:2011-02-19
  • Contact: Zhang Shu-biao, Doctor, Professor, Key Laboratory of State Ethnic Affairs Commission-Ministry of Education, Dalian Nationalities University, Dalian 116600, Liaoning Province, China zsb@dlnu.edu.cn Zhou Ji-ti, Doctor, Professor, School of Environmental and Biological Science, Dalian University of Technology, Dalian 116024, Liaoning Province, China zji@dlut.edu.cn
  • About author:Wang Bing☆, Studying for doctorate, Lecturer, School of Environmental and Biological Science, Dalian University of Technology, Dalian 116024, Liaoning Province, China; Key Laboratory of State Ethnic Affairs Commission-Ministry of Education, Dalian Nationalities University, Dalian 116600, Liaoning Province, China wangbing@dlnu.edu.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 20876027*; Youth Foundation of Dalian Nationalities University, No. 2008A202*

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Abstract:

BACKGROUND: Compared with viral vector, many cationic lipids naturally occurred or synthesized have been used for gene transfer in the form of liposomes, which have the advantages of non-immunogenicity, simple production, plasmid protected against nuclease degradation and non-oncogenicity, etc. And cationic lipids as an effective alternative of viral vector, cationic liposomes can be used for cell transfection in vivo and in vitro.
OBJECTIVE: To introduce research progress on mechanisms of cationic liposome-mediated gene transfer.
METHODS: CNKI database from 1987 to 2010 and PubMed database from 1987 to 2010 were retrieved by the first author with computer. The index words were “gene therapy, cationic liposome, gene transfer, mechanism” in Chinese and English. Literatures were limited to Chinese and English languages. From cationic liposome gene transfer and gene transfer mechanisms were summarized, the cationic liposome-mediated gene transfer mechanisms were reviewed.
RESULTS AND CONCLUSION: Totally 108 literature were retrieved and selected according to inclusion and exclusion criteria, 20 of them were included. The cationic liposome-mediated gene transfer mechanisms were reviewed, including the formation of the cationic lipid / DNA complexes, cell uptake, inclusion body release, complex dissolution, and nucleus intake research. The results indicated that research on structure-activity relationship of lipids and the mechanism of gene transfer is the key to improve cationic liposome transfection efficiency and optimize gene therapy.

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