Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (8): 1449-1453.doi: 10.3969/j.issn.1673-8225.2011.08.029

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Cryopreservation of microencapsulated cells

Ye Ping1,2, Chang Zhao-hua1,2, Liu Bao-lin1,2, Zhao Qing-xiao1,2, Peng Cheng-hong3,4, Shen Bai-yong3,4, Han Bao-san3,4,5   

  1. 1Institute of Refrigeration and Cryogenics, 2Institute of Biology and Thermal Science, College of Medical Devices and Food, University of Shanghai for Science and Technology, Shanghai  200093, China; 3Department of General Surgery, 5Breast Disease Diagnosis and Treatment Center, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai   200025, China; 4Shanghai Institute of Digestive Surgery, Shanghai  200025, China
  • Received:2010-08-24 Revised:2010-09-25 Online:2011-02-19 Published:2011-02-19
  • Contact: Han Bao-san, Doctor, Associate chief physician, Department of General Surgery, Breast Disease Diagnosis and Treatment Center, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; Shanghai Institute of Digestive Surgery, Shanghai 200025, China hanbaosan@126.com
  • About author:Ye Ping☆, Studying for doctorate, Institute of Refrigeration and Cryogenics, Institute of Biology and Thermal Science, College of Medical Devices and Food, University of Shanghai for Science and Technology, Shanghai 200093, China iamyeping@163.com
  • Supported by:

    the National “Eleventh Five-Year” High-Tech Research and Development Program (863 Project), No.2008AA02Z417*; the National Natural Science Foundation of China, No. 30801107*, 30772105*, 30672043*, 20074031*; Key Program of the National Natural Science Foundation of China, No. 20434030*; Scientific Research and Innovation Project of Shanghai Municipal Education Department, No. 09YZ84*; Natural Science Foundation of Shanghai Science and Technology Commission, No. 07ZR14076*, 09140902300*; Science and Technology Research Program of Shandong Province, No. 2008GG10002027*; Graduate Innovation Fund of Shanghai, No. JWCXSL1021*

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Abstract:

BACKGROUND: Microcapsule is an effective tool for immunoisolation, cryopreservation makes the preservation and the transportation of the microcapsules easy, which is helpful for microencapsulated technology applied in clinical practice.
OBJECTIVE: To summarize the cryopreservation of microencapsulated cells, such as cryopreservation characteristic of microencapsulated cells, advantages and disadvantages of different cryopreservation methods, research methods and so on.
METHODS: A computer-based online search of PubMed database (from 1980 to 2010), Wanfang database (from 1991 to 2010) and Vip database (from 1991 to 2010) was performed for articles related to the cryopreservation of microencapsulated cells, cryopreservation technology and devices, cryopreservation application in medical field.
RESULTS AND CONCLUSION: The cryodamage characteristic of microencapsulated cells are different to the cells and tissues. The relatively large size of microcapsules and complex structure of semi-permeable membrane make it susceptibly that microcapsule structure and cells in microcapsules are damaged by ice crystals and solution in cryopreservation. So the cryopreservation protocol of cell suspension could not be used in microencapsulated cells cryopreservation. Slow-cooling and vitrification are two common methods of the cryopreservation of microencapsulated cells, each has advantages and disadvantages. The technology of cryopreservation of microencapsulated cells is not yet mature. The mechanism underlying the cryopreservation of microencapsulated cells needs further strengthen at the same time of exploring the optimal cryopreservation protocols for microencapsulated cells and new cryopreservation facilities for microencapsulated cells.

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