Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (8): 1433-1436.doi: 10.3969/j.issn.1673-8225.2011.08.025

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Ethanol extraction from connecting bones and reducing swelling powder by orthogonal design method

Zhou Yu1, Huang Guo-zhi1, Liang Dong-hui2, Deng Hong-zhu3, Yi Yan-kui3, Zeng Qing1, Wu Wei-wei1, Fan Tao1   

  1. 1Department of Rehabilitation Medicine, 2Department of Traditional Chinese Medicine, Zhujiang Hospital of Southern Medical University, Guangzhou   510282, Guangdong Province, China; 3College of Traditional Chinese Medicine, Southern Medical University, Guangzhou   510515, Guangdong Province, China
  • Received:2010-08-02 Revised:2010-09-25 Online:2011-02-19 Published:2011-02-19
  • Contact: Liang Dong-hui, Chief physician, Professor, Department of Traditional Chinese Medicine, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China dhliang@hotmail.com
  • About author:Zhou Yu★, Studying for master’s degree, Department of Rehabilitation Medicine, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China 1061302248@qq.com
  • Supported by:

    Science and Technology Plan of Guangdong Province, No. 2006B35802005*

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Abstract:

BACKGROUND: The drug effect does not show apparently through the method of aqueous extraction for the connecting bones and reducing swelling powder. The reason could be that the effective components cannot be extracted sufficiently.
OBJECTIVE: To optimize the ethanol extraction technology from the powder of connecting bones and reducing swelling by orthogonal trial.
METHODS: The ethanol extraction rate in prescription and Naringin volume, which is an effective component of principal drug rhizoma drynariae, were taken as the measurement index, for the optimization through L9(34) orthogonal trial. Naringin content was determined by high-performance liquid chromatography method and the ethanol extraction techniques from connecting bones and reducing swelling powder were optimized. The ethanol extraction rate, Naringin volume, and the rate of alcohol absorption were observed
RESULTS AND CONCLUSION: The percentages of ethanol extraction rate and Naringin volume were more than 10% in the third ethanol extraction. The components of Chinese herbal medicine can be completely extracted through three ethanol extractions. The average of alcohol absorption rate was 265.67 mL/g, which means more than 3 times of menstruum would be added in the first ethanol extraction. The result of orthogonal test showed that the dosage of menstruum was the key factor, the ethanol volume fraction was the subordination factor and the time cost in extraction was the most subordination factor, assayed by range R value. The analysis of variance showed that there were significant differences in the effect of the ethanol volume fraction and the dosage of menstruum (P < 0.05), so the optimized extraction condition was: 65% ethanol refluxed and extracted for three times, six times of ethanol extracted for 2 hours, five times for 1.5 hours, five times for 1.5 hours respectively. The optimized extraction technique is superior to routine technology regarding extracting drug components, thus avoiding unwanted technology process. The optimized process is repeatable, simply operated and could reach high naringin content.

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