Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (8): 1429-1432.doi: 10.3969/j.issn.1673-8225.2011.08.024

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Effect of low frequency ultrasound on percutaneous absorption of diclofenac sodium in vitro

Hu Zhi-gang1, Wang Xin-zheng1, Feng Kun2, Jie Xiao-yang3   

  1. 1College of Mechanical and Electrical Engineering, Henan University of Science and Technology, Luoyang   471003, Henan Province, China
    2Experimental Center of Luoyang Orthopedic- Traumatological Hospital, Luoyang   471000, Henan Province, China
    3College of Medical Tehnology Engineering, Henan University of Science and Technology, Luoyang 471003, Henan Province, China
  • Received:2010-09-04 Revised:2010-11-10 Online:2011-02-19 Published:2011-02-19
  • Contact: Wang Xin-zheng, Studying for master’s degree, College of Mechanical and Electrical Engineering, Henan University of Science and Technology, Luoyang 471003, Henan Province, China
  • About author:Hu Zhi-gang☆, Doctor, Associate professor, Master’s supervisor, College of Mechanical and Electrical Engineering, Henan University of Science and Technology, Luoyang 471003, Henan Province, China
  • Supported by:

    the National High-Tech Research and Development Program of China (863 Program), No. 2008AA040208*

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Abstract:

BACKGROUND: Diclofenac sodium is common used on clinical as a powerful non-steroid anti inflammatory and antalgic drug. Gastrointestinal tract degradation and first-pass effect could be avoided after transdermal delivery. But because of the barrier of skin, transdermal drug delivery is limited. The application of low-frequency ultrasound can promote the drug percutaneous absorption by increasing the permeability of the skin.
OBJECTIVE: Based on the study of the influence on percutaneous absorption of diclofenac sodium by low frequency ultrasound, the optimum parameters of diclofenac sodium under low frequency ultrasound were determined.
METHODS: Thirty male rats were randomly divided into ten groups, nine of them were used for orthogonal experiment while one for control experiment without ultrasound. The backside skins of rats were selected for transdermal test. The orthogonal experiment was performed in vitro with ultrasonic frequency, intensity and duration as influencing factors, penetration amount as index, and each parameter of low frequency ultrasound was optimized at the optimal permeation effect of diclofenac sodium, and compared with blank group to observe its significance.
RESULTS AND CONCLUTION: Ultrasonic intensity, frequency, especially duration had very important influence on the increase in the penetration amount. The optimized parameter combination obtained by orthogonal design was composed of ultrasonic frequency (20 kHz), intensity (0.75 W/cm2) and duration (15 minutes). The frequency, intensity, duration of low frequency ultrasound have a significant effect on the penetration amount of diclofenac sodium (P < 0.01). By the comparison of three parameters, ultrasonic duration is the most important.

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