Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (25): 4636-4640.doi: 10.3969/j.issn.1673-8225.2010.25.019

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In vitro biocompatibility between vascular endothelial growth factor/nanohydroxyapatite collagen slow-release system and human bone marrow mesenchymal stem cells

Xu Xiao-feng1, Liu Xiao-ping2, Wang Ming-wei2, Liu Jin-lian3, Chen Jing-jia2, Li Yang2, Zhang Zhi-jian2   

  1. 1 Department of Orthopedics, Affiliated Hospital of Jiangsu University, Zhenjiang  212001, Jiangsu Province, China; 2 School of Medicine, Jiangsu University, Zhenjiang   212013, Jiangsu Province, China; 3Department of Orthopedics, Suzhou Kowloon Hospital, Shanghai Jiao Tong University Medical School, Suzhou   215021, Jiangsu Province, China
  • Online:2010-06-18 Published:2010-06-18
  • About author:Xu Xiao-feng, Chief physician, Associate professor, Master’s supervisor, Department of Orthopedics, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, Jiangsu Province, China aabblxp@163.com
  • Supported by:

    the Social Development Program of Zhenjiang, No. SH2002019*

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Abstract:

BACKGROUND: Using carrier or slow-release system to load growth factor can protect the biological activity of the growth factor and slow down its release, which enhances the cell growth and tissue repairing, regeneration constantly. This is one direction to the exploratory development of controlled release carrier.   
OBJECTIVE: To observe the effect of vascular endothelial growth factor (VEGF)/nano-hydroxyapatite collagen (nHAC) slow-release system and the in vitro biocompatibility with human bone marrow mesenchymal stem cells (MSCs).
METHODS: MSCs obtained from human bone marrow were induced, proliferated in vitro and seeded onto the nHAC scaffolds loaded with VEGF, heparin and fibronectin (experimental group) or single nHAC scaffolds (control group). The quantity and the duration of VEGF release from the scaffolds which loaded with VEGF were determined; the attachment rate of cells on the scaffolds, number of cells in the scaffolds, and alkaline phosphatase (ALP) activity at different time points (3, 7, 10, 14 days) were observed. The cell growth on scaffolds was also observed.
RESULTS AND CONCLUSION:  ①The release of VEGF on the scaffolds of experimental group could last for 14 days. ②The differentiation of MSCs into osteoblastic phenotype was demonstrated by the positive staining of alkaline phosphatase (ALP) and collagen type I. ③The adhesive rates, cell numbers and the expression of the ALP at the same time point in the scaffolds of experimental group were significantly greater compared with control group (P < 0.05). ④Cells could be observed on every scaffold by scanning electron microscopy, and the cells in experimental group grew better than the control group.

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