Abstract:

BACKGROUND: Local drug controlled-delivery systems for anti-inflammatory are gaining increasing attention for bone defect regeneration because of their extended therapeutic effects and reduced adverse effects. Electrospun polymer nanofiber is a kind of good drug delivery vehicle.
OBJECTIVE: To fabricate tetracycline hydrochloride-loaded polyglycolic acid (PLGA) nanofibrous membrane with varied tetracycline hydrochloride contents using the electrospinning technique, and to study their performance in preventing bacterial infections as well as their cell compatibility.
METHODS: Tetracycline hydrochloride-loaded PLGA nanofibrous membranes were synthesized at 15-20 kV under a steady flow rate of 0.3-0.5 mL/h using an electrospinning technique. Through the images of scanning electron microscopy, we obtained morphological characters of the nanofibers. Drug entrapment efficiency was determined, drug release profiles were tested and in vitro antimicrobial activities were observed from modified Kirby-Bauer test. MG-63 cells were used to test the biocompatibility of nanofibrous membrances.
RESULTS AND CONCLUSION: Average diameters of PLGA nanofibers with different tetracycline hydrochloride contents (0%, 3%, 5% or 10%) were all about 360-470 nm. Tetracycline hydrochloride entrapment efficiencies were well above 80% for all the PLGA membranes. The 10% drug loaded membranes exhibited the highest initial burst release. Tetracycline hydrochloride-loaded PLGA nanofibrous membranes were effective in inhibiting growth of staphylococcus aureus but had no adverse effect on the adhesion and proliferation of MG-63 cells. Comparatively speaking, electrospun PLGA/tetracycline hydrochloride membranes with drug loading of 3% and 5% are good choices for the prevention of bacterial infections in guided tissue regeneration.