Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (25): 4623-4626.doi: 10.3969/j.issn.1673-8225.2010.25.016

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Chitosan and collagen gel constructed tissue-engineered oral mucous propria

Ding Yue1, Wu Zhi-qiang2, Zhang Li-ping2   

  1. 1 Department of Stomatology, People’s Hospital of Liaoning Province, Shenyang  110015, Liaoning Province, China; 2 Department of Stomatology, Shengjing Hospital of China Medical University, Shenyang  110004, Liaoning Province, China
  • Online:2010-06-18 Published:2010-06-18
  • About author:Ding Yue★, Master, Attending physician, Department of Stomatology, People’s Hospital of Liaoning Province, Shenyang 110015, Liaoning Province, China dd120_cool@126.com
  • Supported by:

     the Scientific Research Foundation of Liaoning Provincial Science and Technology Department, No. 00225001-5*

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Abstract:

BACKGROUND: Studies have explored the feasibility of tissue engineered artificial human skin using chitosan-collagen as scaffold material.
OBJECTIVE: To investigate the feasibility of in vitro construction of artificial lamina propria using artificial scaffold of chitosan-collagen and oral mucous fibroblasts (OMF).
METHODS: The OMF was extracted from adult rat buccal division, gingival or pars palatalis and cultured in 2XDMEM solution, serum of embryo cattle, collagen solution and chitosan, or 2XDMEM solution, serum of embryo cattle and collagen solution were mixed together into gel solution pro rata. OMFs were added into above gel solution to form oral mucous of artificial lamina propria. OMFs were observed; chitosan-collagen gel substrate and collagen gel substrate were also investigated.
RESULTS AND CONCLUSION: After 1 day of collagen-OMF complex (FPCL) and chitosa-collagen-OMF complex (FPCCL) culture, OMF adhered to artificial scaffold and grew in rendered olive-like and unordered. OMFs proliferated with time, and the gel became pale and thin, constrict at second and third days respectively. OMFs may grow and secrete substrate in three-dimensional space formed by chitosan-collagen gel, which forms compact connective tissue similar to oral mucous propria. Chitosan-collagen gel is a suitable material for constructing oral mucous propria by tissue engineering.

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