Abstract:

BACKGROUND: Traditional porcine dermal dressing can protect wounds, but it has not the properties of bacteriostasis efficacy.
OBJECTIVE: To assess in vitro toxicity of self-made nano-silver porcine acellular dermal dressing and to evaluate the feasibility of a new type of dressing.
METHODS: L-929 mouse fibroblasts were used to detect the cell relative proliferation rate in the extract from the nano-silver porcine dermal dressing 100% and 50% by MTT-assay, the toxicity of porcine dressing was also determined. Morphology of the cells was observed under inverted microscope. The absorbance value was detected using enzyme-linked immunospot assay at 2, 4, 7 days of the culture, and the cytotoxicity was assessed.
RESULTS AND CONCLUSION: The L-929 cells proliferated well in the extract of nano-silver porcine acellular dermis dressing, and the cell toxicity was graded as 1. There was no significant difference in the absorbance value between the experimental group and the negative control group ( P > 0.05). The nano-silver pig acellular dermis dressing shows no evident cytotoxicity and is feasible to serve as a new type of wound dressing.