Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (25): 4599-4602.doi: 10.3969/j.issn.1673-8225.2010.25.010

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Growth and proliferation of preadipocyte seeded on small intestinal submucosa

Lan Hai 1,2, Huang Fu-guo1, Yang Zhi-ming1, Luo Jing-cong1, Li Xiu-qun1   

  1. 1 Department of Orthopaedics, West China Hospital, Sichuan University, Chengdu   610041, Sichuan Province, China; 2 Department of Orthopaedics, Chengdu Railway Center Hospital, Chengdu   610081, Sichuan Province, China
  • Online:2010-06-18 Published:2010-06-18
  • Contact: Huang Fu-guo, Master, Professor, Department of Orthopaedics, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China hfg2009@163.com
  • About author:Lan Hai☆, Doctor, Department of Orthopaedics, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China; Department of Orthopaedics, Chengdu Railway Center Hospital, Chengdu 610081, Sichuan Province, China sealan2000@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30270398*

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Abstract:

BACKGROUND: The preadipocytes could differentiate into adipocyes with strong proliferation capacity. Small intestinal submucosa (SIS) has good biocompatibility, could be suitable scaffold for tissue engineering.
OBJECTIVE: To culture preadipocytes in vitro on SIS, and to study the growth and proliferation of preadipocytes on SIS.
METHODS: The preadipocytes cultured were respectively seeded on SIS and pore plates, serving as material group and control group. Cell number in different periods was determinded by MTT, and the growth curve was rendered. The proliferation capacity of the preadipocytes was observed under different culture conditions. Flow cytometry was used to detect the proliferation and apoptosis of preadipocytes seeded on SIS and pore plates. Preadipocytes on SIS were also observed for the growth and proliferation at different time points.
RESULTS AND CONCLUSION: The growth curve showed that, the preadipocytes on SIS grew rapidly and the cell number was higher than that in pore plates on day 13 (P < 0.05), suggesting a strong proliferation capacity. Cells on SIS exhibited stronger proliferative capacity than those in pore plate (P < 0.05). Apoptotic rates were low in two groups without statistical difference. The preadipocytes are shown to well adhere and grow on SIS. SIS is very biocompatible with preadipocytes.

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