Abstract:

BACKGROUND: Polymer based materials can improve surface chemical structure via increasing inside inorganic material. A series of techniques have been produced and developed for synthesizing biodegradable materials, and supercritical fluid technique is a superior one.
OBJECTIVE: To substitute biomaterials by a composite bone fabricated by supercritical fluid technique, and to evaluate the biological characteristics of the novel composite biomaterial.
METHODS: Granulated allogeneic bone and polymer of lactic acid (PLA) were compounded to fabricate the composite scaffold by the supercritical fluid technique. The composite scaffold was cytological estimated through on osteoblasts line culture. Meanwhile the sensitization and tissue inflammation were observed by animal experiment that the saturation was injected subcutaneously and the scaffold was implanted directly into muscle.
RESULTS AND CONCLUSION: The figuration, volume and micro-structure of the scaffold were controllable and the biological characteristics of the novel composite biomaterial were excellent. In vivo test demonstrated that, no sensitization response occurred, but the material had no ability of heterotopic osteogenesis. The results illustrated that the prepared novel composite biomaterial can meet the requirement of cellular compatibility and biocompatibility, which has a broad prospect in future.