Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (25): 4573-4576.doi: 10.3969/j.issn.1673-8225.2010.25.004

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Osteogenesis of an injectable tissue-engineered bone monitored by 99Tcm-MDP bone scintigraphy

Huang Ruo-kun, Xie Ming, Fang Zhen-hua, Kan Wu-sheng, Cheng Wen-jun, Hu Jia-lang   

  1. Department of Foot & Ankle Surgery, Puai Hospital of Tongji Medical College of Huazhong University of Science and Technology, Wuhan   430033, Hubei Province, China
  • Online:2010-06-18 Published:2010-06-18
  • About author:Huang Ruo-kun★, Master, Department of Foot & Ankle Surgery, Puai Hospital of Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430033, Hubei Province, China huangrk@126.com

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Abstract:

BACKGROUND: Osteogenesis has been commonly observed by ultrasound, X-ray and CT, but nuclide monitor is rarely reported.
OBJECTIVE: Using 99Tcm-medronate (MDP) bone scintigraphy to monitor the improvement of osteogenesis in the distracted gap by an injectable tissue-engineered bone to assess the value of radionuclide for monitoring the therapeutic effect of this approach.
METHODS: A total of 20 rabbits were divided into experimental and control groups. A 20 mm incision was made in the distal tibia shaft in all rabbits in addition to the metaphysis osteotomy, and stabilized with an self-designed mini external fixator bilaterally. From post-operative day 7, bone fragments were distracted by 1 mm a day. Autologous bone marrow mesenchymal stem cells were isolated and the second passage was used. After elongation, bone marrow mesenchymal stem cells and platelet rich plasma were percutaneously injected in the distracted gap of experimental group, while the control group was injected with the same volume of normal saline. Radionuclide was used to monitor the therapeutic effects at 2, 4, and 8 weeks.
RESULTS AND CONCLUSION: Radionuclide accumulation was observed in the distraction area in two groups after 3 hours of 99Tcm-MDP injection, which was stronger compared with the healthy side. The bone/soft tissue contrast was clear, and symmetric radioactive increase areas were detected at the big joint and tendon. 99Tcm-MDP uptakes and the target to non-target ratios (T/NT) indicated an increasing tendency of radionuclide accumulation in the distracted gap of experimental group and control group at 2, and 4 postoperative weeks. There was a decreasing tendency of radionuclide accumulation in experimental and control groups at 8 weeks, but the differences between two groups were statistically significant (P < 0. 01). Results show that percutaneous injection of injectable tissue-engineered bone could improve osteogenesis in the distracted gap, and radionuclide imaging may accurately monitor the new bone formation and maturation in the early phase.

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