Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (25): 4568-4572.doi: 10.3969/j.issn.1673-8225.2010.25.003

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Biological properties of demineralized bone/polylactic acid composite artificial bone in vivo

Liu Jin-biao, Huang Gang, Pan Xian-ming, Ma Ze-hui, Deng Bing, Deng Shao-lin, Zhang Bo, Zhou Jin-song, Wu Hong-hua   

  1. Department of Orthopaedics, General Hospital of Chengdu Military Area Command of Chinese PLA, Chengdu  610083, Sichuan Province, China
  • Online:2010-06-18 Published:2010-06-18
  • About author: Liu Jin-biao, Master, Associate chief physician, Department of Orthopaedics, General Hospital of Chengdu Military Area Command of Chinese PLA, Chengdu 610083, Sichuan Province, China liujinbiao@medmail.com.cn
  • Supported by:

    a Grant of General Hospital of Chengdu Military Area Command of Chinese PLA, No. 03Y007*

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Abstract:

BACKGROUND: Previous studies have exhibited that demineralized bone/polylactic acid composite artificial bone (BPCB) meets the requirements of artificial bone material in its porosity rate, pore size, mechanical strengths and possess both quality of demineralized bone and polylactic acid. BPCB can facilitate the adhension and proliferation of human osteoblasts significantly.
OBJECTIVE: To further investigate compatibility, degradation and bone formation of BPCB in vivo.
METHODS: Bone defects with 1.5 cm in length were filled with BPCB or nothing randomly soon after they were produced at both radii of adult New Zealand rabbits. Furthermore, BPCB granules were embedded in the same side gluteus. The animals’ local and whole body response to the implants were observed after the operation, and their serum calcium levels were detected. At 6, 8, 12 and 16 weeks postoperation, samples at bone defect region were evaluated by X-ray, bone mineral content (BMC), general sample and underwent histomorphological observation. Samples from gluteus only received histomorphological observation. Tissues response, new bone formation in the defects and degradation of the implants were analyzed at different phases.
RESULTS AND CONCLUSION: Following BPCB transplantation, no significant local adverse reactions and no significant changes in serum calcium levels were found. BMC in the defects following BPCB grafts was increased more rapidly than the blank control group during 6-16 weeks. Gross, X-ray and histological observations revealed that BPCB was osteoconductive. At 16 weeks, the defects nearly got completely repaired while the blank control group remained nonunion. Biodegradation of the implants was observed early following implantation, and there were many macrophages and multinuclear giant cells swallowed with particles of BPCB surrounding the implants at 12 weeks, but there were still remnants of BPCB in the defects at 16 weeks. BPCB possesses good biocompatibility, bone conduction and bone formation ability, and biodegradation occurs.

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