Abstract:

BACKGROUND: MG-132 is a kind of proteasome inhibitor, which has delaying effect on atrophy of denervated skeletal muscle.
OBJECTIVE: To detect the effect of proteasome inhibitor MG-132 on expression of myogenic regulatory factors Myf-5 gene of denervated skeletal muscle in rats.
METHODS: Sprague-Dawley rats were randomly divided into three groups. Sham operation were made only for the rats in the control group (sciatic nerves were not cut off). Sciatic nerves were cut off more than 1-cm at the mid-level on their right lower limbs for the rats in the denervated and MG-132 intervention groups. Rats were sacrificed at 2, 7, and 28 days after operation. The expression of Myf-5 mRNA was detected by RT-PCR, and the changes of myf-5 protein were determined by Western blot.
RESULTS AND CONCLUSION: Compared with the control group, the expressions of Myf-5 mRNA and protein were increased in the denervated and MG-132 intervention groups at 2, 7, and 28 days after operation (P < 0.01). The increasing of Myf-5 mRNA in the MG-132 intervention group was greater than that in the denervated group (P < 0.01). The results suggested that the proteasome inhibitor MG-132 can inhibit the ubiquitin-proteasome pathway to increase the expression of Myf-5 gene in order to play the role of slowing skeletal muscle atrophy.