Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (24): 4473-4476.doi: 10.3969/j.issn.1673-8225.2010.24.023

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Expression of caspase-3/8 mRNA in hypertrophic scars

Zhu Shao-ban1, Liu Da-en1, Li Hong-mian2, Niu Hai-yan3   

  1. 1 Department of Burn and Plastic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China; 2 Department of Plastic and Aesthetic Surgery, Affiliated Zhongshan Hospital of Sun Yat-sen University, Zhongshan  518403, Guangdong Province, China; 3 Department of Pathology, Southern Medical University,  Guangzhou   510515, Guangdong Province, China
  • Online:2010-06-11 Published:2010-06-11
  • Contact: Liu Da-en, Chief physician, Department of Burn and Plastic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China Liudaen@163.com
  • About author:Zhu Shao-ban★, Master, Physician, Department of Burn and Plastic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China Shaoban303@163.com
  • Supported by:

    the Natural Science Foundation of Guangxi Zhuang Autonomous Region, No. 0728110*

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Abstract:

BACKGROUND: Apoptosis participates in the dynamic process of cell component decease due to granulation tissues transforming into scar tissues, and Caspase family mediated apoptosis is the main pathway for apoptosis. Severe burns often reduces the formation of hypertrophic scars, Seriously affected in patients with body function and aesthetic, the treatment of hypertrophic scars are still exploring at the molecular level.
OBJECTIVE: To explore the role of caspase-3 mRNA and caspase-8 mRNA in hypertrophic scars at the gene level.
METHODS: Pathological scar tissue and normal skin tissue were obtained from 18 patients who received treatment at the Department of Burn and Plastic Surgery, the First Affiliated Hospital of Guangxi Medical University. Additional normal skin tissues served as controls. The expression of caspase-3 mRNA and caspase-8 mRNA in hypertrophic scars was tested by semi-quantitative reverse polymerase chain reaction method.
RESULTS AND CONCLUSION: The expression rate of caspase-3 mRNA and caspase-8mRNA in hypertrophic scar were (8.31±1.47) and (7.12±1.24), respectively, which were significantly smaller than those of the normal skins (P < 0.05). The results indicated that caspase-3 mRNA and caspase-8 mRNA might play an important role in the process of wound healing and that the formation of hypertrophic scar might be due to the down-regulation of caspase-3 mRNA or (and) caspase-8 mRNA expression.

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