Abstract:

BACKGROUND: Adiponectin plays a regulatory role in insulin resistance and metabolic syndrome, and the synthesis of which is regulated by peroxisome proliferator-activated receptor γ (PPAR γ). Studies demonstrated that angiotensin receptor blocker  can affect the metabolism of adiponectin via activating PPAR γ, but the precise mechanism remains unclear.
OBJECTIVE: To investigate the effect of telmisartan on the adiponectin expression in 3T3-L1 adipocytes in vitro, and to compare the results with candesartan.
METHODS: 3T3-L1 adipocytes were cultured and differentiated in vitro. The differentiated 3T3-L1 adipocytes were cultured with vehicle or candesartan (10 μmol/L) or telmisartan (10 μmol/L). The expression level and secretary level of adiponectin were detected by RT-PCR, Western blot and ELISA.
RESULTS AND CONCLUSION: Compared with the control and candisartan groups, telmisartan significantly increased the expression of adiponectin mRNA and protein in adipocytes (P < 0.05, P < 0.01), but there was no significant difference in secretion levels of adiponectin between groups (P > 0.05). The results demonstrated that telmisartan can increase the adiponectin expression in 3T3-L1 adipocytes, but can not increase the secretion by 3T3-L1 adipocytes. The promotive effect of telmisartan is superior to candesartan.