Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (21): 3865-3868.doi: 10.3969/j.issn.1673-8225.2010.21.015

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Loading and release of lysozyme by AMS-8 mesoporous silica

Zhang Qi1, Zhang Zhuo-qi2, Chen Min-min1, Meng Yun-jun1, Cao Xi-chuan1   

  1. 1 School of Materials Science and Engineering, China University of Mining and Technology, Xuzhou  221116, Jiangsu Province, China; 2 Department of Cardiology, the Affiliated Hospital of Xuzhou Medical College, Xuzhou   221002, Jiangsu Province, China
  • Online:2010-05-21 Published:2010-05-21
  • Contact: Cao Xi-chuan, Professor, Doctoral supervisor, School of Materials Science and Engineering, China University of Mining and Technology, Xuzhou 221116, Jiangsu Province, China xichuancao@yahoo.com.cn
  • About author:Zhang Qi★, Studying for master’s degree, School of Materials Science and Engineering, China University of Mining and Technology, Xuzhou 221116, Jiangsu Province, China zqcumt049@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30670557*; the National Natural Science Foundation of Jiangsu Province, No. BK2008132*

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Abstract:

BACKGROUND: Mesoporous materials have high specific surface area, uniform and controlled pore size distribution, regular pore structure and strong adsorption capacity, it is a good enzyme immobilization carrier. Current studies of AMS materials mainly focus on the synthesis and development of new materials, while few reports are abont the adsorption and release behavior of biological molecules.
OBJECTIVE: To screen the best conditions for mesoporous material AMS-8 adsorbing and releasing lysozyme, and to observe the enzyme adsorption and releases in different conditions.
METHODS: Mesoporous material AMS-8 was synthesized with hydrothermal method using anionic surfactant and co-structure-directing agents such as 3-aminopropyltrimethoxysilane. The materials were characterized consecutively by powder X-ray diffraction and N2 adsorption/desorption analysis. The initial concentrations of enzyme solution were changed to determine adsorption changes, the release changes were measured in PBS eluents at different pH values.
RESULTS AND CONCLUSION: The calcined AMS-8 have ordered cubic cage-type mesoporous structure. The BET surface areas were 867 m2/g and the pore diameter was 3.4 nm. Lysozyme was selected as a model molecule to observe the absorption behavior of AMS-8 to lysozyme molecule under different concentrations at pH7.0. Lysozyme adsorption study suggested that the highest loading capacity was 136 mg/g at the initial concentration of 0.5 g/L. The release profile of lysozyme/AMS-8 system to lysozyme at different pH values was also investigated. The results showed that mesoporous materials AMS-8 exhibited a good sustained-release effect, and sustained manner is controlled by pH value.

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