Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (21): 3847-3850.doi: 10.3969/j.issn.1673-8225.2010.21.011

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Preparation of chitosan/konjac glucomannan composite membranes and the in vitro performances

Duan Kai-wen1, Zhao Li-fen2, Li Cheng-wen3, Chen Qing-hua4, Ma Li-li1, Li Na-na4   

  1. 1 Department of Stomatology, Affiliated Yan’an Hospital of Kunming Medical University, Kunming  650051, Yunnan Province, China; 2 Haiyuan School of Kunming Medical College, Kunming  650021, Yunnan Province, China; 3 Yunnan Medical College, Kunming  650021, Yunnan Province, China; 4 College of Material and Metallurgical Engineering, Kunming University of Science and Technology, Kunming  650093, Yunnan Province, China
  • Online:2010-05-21 Published:2010-05-21
  • About author:Duan Kai-wen☆, Doctor, Professor, Department of Stomatology, Affiliated Yan’an Hospital of Kunming Medical University, Kunming 　650051, Yunnan Province, China kwduan@yahoo.com.cn

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Abstract:

BACKGROUND: Recent studies indicated that the composition of chitosan (CS) and konjac glucomannan (KGM) can improve their own biological features.
OBJECTIVE: To prepare CS / KGM composite membranes, and to evaluate its effect on the proliferation of periodontal ligament fibroblast cells (PDLCs).
METHODS: Blend membranes containing CS and KGM solutions with different weight proportions were prepared by solvent-casting method. The 6th passage of human umbilical cord mesenchymal stem cells were used to observe the cell compatibility and cytotoxicity and to determine the degradation features. The effects of those cells on PDLCs were assessed.
RESULTS AND CONCLUSION: Eight kinds of composite membranes containing CS and KGM (3:0, 2:1, 1:1, 1:2) were obtained. In vitro degradation test demonstrated that the degradation rates of CS / KGM composite membranes were increased with the adding of KGM, which were 40.75%, 65.70%, 74.20% and 84.60% respectively at 70 days. In vivo test showed that the CS / KGM composite membranes could maintain more than 12 weeks when at the CS / KGM ratios of 3:0, 2:1 and 1:1. If the CS / KGM ratio was 1:2, the CS / KGM composite membranes could maintain 8 weeks in vivo. The effects of the composite membranes on human PDLCs were decreased with increasing of KGM contents. After adding basic fibroblast growth factor, the CS / KGM composite membranes with ratios of 3:2 and 2:1 could increase the alkaline phosphatase activities, especially with ratio of 2:1. Results from the study suggested that CS / KGM membrane is a potential periodontal tissue engineering scaffold.

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