Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (21): 3827-3831.doi: 10.3969/j.issn.1673-8225.2010.21.007

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Effect of basic fibroblast growth factor on adhesion and proliferation of Schwann cells on small intestinal submucosa scaffold

Zhang Kai-wei1, Xiao Rui2, Duan Hong1, Xiang Zhou 1,3, Li Xiu-qun3   

  1. 1 Department of Orthopaedics, West China Hospital, Sichuan University, Chengdu   610041, Sichuan Province, China; 2 Department of Orthopaedics, the First People’s Hospital of Yibin, Yibin  644000, Sichuan Province, China; 3 Division of Stem Cells and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu   610041, Sichuan Province, China
  • Online:2010-05-21 Published:2010-05-21
  • Contact: Xiang Zhou, Doctor, Professor, Chief physician, Doctoral supervisor, Department of Orthopaedics, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China; Division of Stem Cells and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China xiangzhou15@hotmail.com
  • About author:Zhang Kai-wei☆, Studying for doctorate, Associate chief physician, Department of Orthopaedics, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China zkw1973@yahoo.com.cn
  • Supported by:

    the Key Science and Technology Development Program of Sichuan Province, No. 200803010*

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Abstract:

BACKGROUND: Schwann cells combined with small intestinal submucosa (SIS) is a feasible way to construct artificial nerve. Basic fibroblast growth factor (bFGF) can promote proliferation of Schwann cells.
OBJECTIVE: To explore the effects of bFGF on adhesion and proliferation of Schwann cells on scaffold of SIS.
METHODS: The second passage of Schwann cells isolated from neonatal SD rats were seeded onto the surface of SIS scaffold and incubated with 50 μg/L bFGF, which served as experimental group. The cultures of SC and SIS without bFGF were used as control. The cell proliferation was measured with MTT assay, and the adhesion of Schwann cells was determined by the rate of cell adhesion; the cell cycle of SC was examined by flow cytometry. The morphology of cells and cell adhesion on SIS were observed by contrast phase microscope, hematoxylin-eosin (HE) staining and scanning electron microscopy.
RESULTS AND CONCLUSION: Absorbance value of SC in experimental group was higher than the control group (P < 0.05). The cell adhesion rate was (69.47±3.17)% and (44.58±1.76)% respectively for experimental and control groups (P < 0.05). G2/M+S percentage of SCs was significantly greater in experimental group than the control group (P < 0.05). HE staining and microscopy showed that the number of attached cells was more and cell adhesion was better in experimental group compared with the control group at 7 days after culture. bFGF is effective in improving the adhesion and proliferation of SCs on SIS scaffold, and also promoting construction of tissue-engineered nerve conduit based on the combination with SC and SIS.

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