Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (21): 3806-3810.doi: 10.3969/j.issn.1673-8225.2010.21.002

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Osteoinductive activity of self-made human bone morphogenetic protein-2 composite bone in bone repairing

Wang Ju1, Xiao Gang1, Chen Yang1, Zhou Zhi-you1, Lin Yong-liang2, Wu Hao3, Zha Zhen-gang3, Zhou Tian-hong1   

  1. 1 College of Life Science and Technology, Jinan University, Guangzhou   510632, Guangdong Province, China; 2 Grandhope Biotechnology Limited Company, Guangzhou   510663, Guangdong Province, China; 3 Department of Orthopaedics, Jinan University Affiliated First Hospital, Guangzhou   510632, Guangdong Province, China
  • Online:2010-05-21 Published:2010-05-21
  • Contact: Zha Zhen-gang, Doctor, Professor, Department of Orthopaedics, Jinan University Affiliated First Hospital, Guangzhou 510632, Guangdong Province, China
  • About author:Wang Ju★, Master, Associate investigator, College of Life Science and Technology, Jinan University, Guangzhou 510632, Guangdong Province, China wangju1688@163.com
  • Supported by:

    Province and Ministry Project of Industry, University and Research, No. 2007A090302083*; Provincial Scientific and Technological Project, No. 2009B030801237*; Enterprise Scientific Special Agents by Guangdong Province, Ministry of Education and Ministry of Science and Technology, No. 2009B090600134*

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Abstract:

BACKGROUND: Bone morphogenetic protein is a kind of growth factors only which can induce bone regeneration independently that we found until now. The prospect of clinical application of bone morphogenetic proteins is very bright. But their high cost limits their use in China.
OBJECTIVE: To evaluate the osteoinductive activity and the bone-defect repairing ability of self-made recombinant human bone morphogenetic protein (rhBMP-2).
METHODS: The purity of rhBMP-2 was assayed by SDS-PAGE and mass spectrography. The osteoblastic to differentiation markers of C2C12 cells after induction of rhBMP-2 were assayed to identify the osteoinductive activity of rhBMP-2. The bone-defect repairing ability of rhBMP-2 was verified by New Zealand rabbit radial bone repair trial after the composition of material and rhBMP-2.
RESULTS AND CONCLUSION: The purity of rhBMP-2 was ≥95%, rhBMP-2 could greatly induce C2C12 cells to differentiate into osteoblasts, alkaline phosphatase largely expressed at early stage of the differentiation, and many mineralized nodules formed at end stage, indicating good osteoinductive activity of the self-produced rhBMP-2. In the New Zealand rabbit radial bone repair trials, the results of X-ray assay and hematoyline-eosin staining showed that, massive callus formed on the surface of rhBMP-2 composite material at 2 months, and fused with autologous bone tissue at 4 months, a large amount of materials degraded. In the pure material group, a small amount of bone bridges produced newly linked with the planted material in which the degradation was little. In blank group, the defect area was filled with fibrosis tissue. The results suggested that self-produced rhBMP-2 could markedly promote the bone repairing and could be used for clinical application.

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