Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (21): 3801-3805.doi: 10.3969/j.issn.1673-8225.2010.21.001

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Repair of the rabbit ulnar bone nonunion through percutaneous injection of autologous red bone marrow and bioactive nano-bone putty complex

Peng Cheng1, Cui Fu-zhai2, Zhu Zhen-an3, Hu Kun2, Sun Da-chuan1, Li Jun-guang1, Hu Chun-lin1, Liu Zong-hai1, Sheng Hong-biao1　   

  1. 1 Department of Orthopaedic Surgery, Fengcheng Branch of Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai  201411, China; 2 Department of Materials Science and Engineering, Tsinghua University, Beijing  100084, China; 3 Department of Orthopaedics, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai  200011, China
  • Online:2010-05-21 Published:2010-05-21
  • About author:Peng Cheng★, Master, Associate chief physician, Department of Orthopaedic Surgery, Fengcheng Branch of Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201411, China pengcheng1111@126.com
  • Supported by:

    the Scientific Research Subject of Health Department of Shanghai Fengxian District, No. 200806*

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Abstract:

BACKGROUND: The development of nanotechnology and the deepening understanding of autogenous bone marrow provide a new idea for repairing bone nonunion.
OBJECTIVE: To discuss the feasibility of repairing bone nonunion through percutaneous injection of autologous red bone marrow and bioactive nano-bone putty complex.
METHODS: New Zealand rabbits were prepared for ulnar bone nonunion models and were randomly divided into 4 groups; the red bone marrow + bioactive nano-bone putty, bioactive nano-bone putty, and enriched red bone marrow were percutaneously injected into the rabbit ulnar bone nonunion. There was no treatment in the blank control group. The repair capability was assessed by radiographic examination, and the gross observation, biomechanics measurement, and histological observation were performed at 12 weeks after operation. 
RESULTS AND CONCLUSION: Radiological assessment showed that the new bone formation was: enriched red bone marrow + bioactive nano-bone putty group > bioactive nano-bone putty group > enriched red bone marrow group > blank control group (P < 0.01). The biomechanics measurement displayed that biomechanical strength of the enriched red bone marrow + bioactive nano-bone putty group was greater than that of the bioactive nano-bone putty group (P < 0.01), but smaller than normal bone tissues (P < 0.01). The histologic check showed that in enriched red bone marrow + bioactive nano-bone putty group, there were a great number of osteoblasts and new mass, and bone union appeared in the place of bone nonunion. In enriched red bone marrow group, few formation of new bone in the place of bone nonunion which finally filled with fibrous tissues. There was no formation of new bone in the place of bone nonunion where fibrous tissues were finally filled in the blank control group. The enriched red bone marrow and bioactive nano-bone putty complex exhibit osteoinductive ability, and has a better bone nonunion repair capability than nanobone putty or red marrow used only regarding the bone formation rate, quality and mechanical strength. The complex graft can repair rabbit ulnar bone nonunion through percutaneous injection.

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