Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (16): 2916-2920.doi: 10.3969/j.issn.1673-8225.2010.16.017

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Cytotoxicity of a new type of antibiotic stainless micro-screw implant

Zhang Dan1, Zhang Yang1, Lu Li2, Yang Ke3, Xue Nan1   

  1. 1 Department of Orthodontics, School of Stomatology, China Medical University, Shenyang  110002, Liaoning Province, China; 2 Department of Oral and Maxillofacial Surgery, School of Stomatology , China Medical University, Shenyang  110002, Liaoning Province, China; 3 Institute of Metal Research, Chinese Academy of Sciences, Shenyang  110016, Liaoning Province, China
  • Online:2010-04-16 Published:2010-04-16
  • Contact: Zhang Yang, Professor, Chief physician, Department of Orthodontics, School of Stomatology, China Medical University, Shenyang 110002, Liaoning Province, China
  • About author:Zhang Dan★, Master, Lecturer, Attending physician, Department of Orthodontics, School of Stomatology, China Medical University, Shenyang 110002, Liaoning Province, China dan20040326@sohu.com

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Abstract:

BACKGROUND: Prevention of implant from inflammation was an effective method to reduce expulsion rate of stainless steel micro-screw implant, and develop new type of antibiotic material.
OBJECTIVE: To evaluate the cytotoxicity of a new type of antibiotic stainless steels.
METHODS: Metal test samples (antibiotic stainless steel, medical stainless steel, and medial pure titanium) were made into rectangular solids with length of 15 mm × 10 mm × 3 mm. Samples were cleaned with high temperature and high pressure. Alloy leaching liquor was prepared with DMEM culture media according to the ratio between surface area and volume of culture solution (3 cm2/mL). The leaching liquor was maintained in incubator at 37 ℃ for 96 hours, and then degerming was performed using microporous membrane. 6.4% phenol was added, which was considered as the positive control group, and DMEM culture media was considered as the blank control group. Growth of MG63 cells was observed under inverted phase contrast microscope; absorbance of cells cultured for 24, 48, 72, 96, and 120 hours was detected using MTT test; cytotoxicity of antibiotic stainless steels was evaluated.
RESULTS AND CONCLUSION:  ① At 24 hours after culture, cells in the positive control group was abnormal; while, cells in other groups were well adherent-grew. ② After 48 hours of culture, with the culture time increased, cytotoxicity was detected out in the positive control group; cells in other groups and blank control groups were normal and grew well. A few of cells in stainless steels group showed karyopyknosis. ③ The absorbance was the highest of medical pure titanium, and then of antibiotic stainless steel and of medical stainless steel, while there was no significant difference between the three materials. ④ The level of cytotoxicity was grade 0. The results suggested that the antibiotic stainless steel which had the same cytotoxicity grade as medical stainless steel and pure titanium was in line with the requirement of its clinical application.

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